ISSN 1674-3865  CN 21-1569/R
主管:国家卫生健康委员会
主办:中国医师协会
   辽宁省基础医学研究所
   辽宁中医药大学附属医院

中国中西医结合儿科学 ›› 2020, Vol. 12 ›› Issue (5): 466-468.doi: 10.3969/j.issn.1674-3865.2020.05.027

• 临床研究 • 上一篇    

痰培养标本采集时间与采集方法对支气管肺炎患儿标本质量的影响研究

蔡恒洋   

  1. 118300 辽宁 东港,东港市中心医院检验科
  • 出版日期:2020-10-25 发布日期:2020-11-28
  • 作者简介:蔡恒洋(1976-),男,副主任技师。研究方向:医学微生物检验,E-mail:caihengyang50109@163.com

Influence of the time and method of collecting specimen in sputum culture on the quality of specimen in children with bronchopneumonia

  • Online:2020-10-25 Published:2020-11-28

摘要: 目的 探讨支气管肺炎患儿痰培养标本不同采集时间和采集方法对标本检验质量的影响。 方法 选择2017年2月至2019年2月我院收治的支气管肺炎患儿150例,随机分为对照组和观察组各75例。观察组采用吸痰法采集标本,第一次采样时间为入院立即采用,第二次采样时间为入院第2天晨起空腹2 h以上采集;对照组采用棉拭子法采集标本,第一次采样时间为入院立即采用,第二次采样时间为入院第2天晨起空腹2 h以上采集。比较不同时间两组痰培养标本检出总菌株数及菌株阳性率、标本退回率、准确率。 结果 观察组不同时间的痰标本培养总菌株检出数及菌株阳性率均高于对照组,两组晨起时的总检出菌株数及阳性率均高于随机时,差异有统计学意义(P<0.05);观察组不同时间痰标本退回率均显著低于对照组,两组晨起时的标本退回率均低于随机时,差异有统计学意义(P<0.05);观察组不同时间痰培养标本准确率均显著高于对照组,两组晨起时的准确率均高于随机时,差异有统计学意义(P<0.05)。 结论 支气管肺炎患儿入院后第2天晨起空腹吸痰法痰培养标本采集,显著提升标本菌株数的检出率,提升检出结果的阳性率和准确率,降低标本的退回率,效果更加显著,具有积极推广价值。

关键词: 支气管肺炎, 痰培养, 标本采集, 采集时间, 采集方法, 标本质量, 精准度, 儿童

Abstract: Objective To study the influence of different time and method of collecting specimen in sputum culture on the detection quality of specimen in children with bronchopneumonia. Methods A total of 150 children with bronchopneumonia were treated in our hospital from Feb. 2017 and Feb. 2019, and they were divided into control group(75 children) and observation group(75 children) by lottery. The specimen was taken by way of sputum aspiration in the observation group: the first time was on admission to hospital and the second time was at 2h fasting on the morning of the second day after admission; in the control group, the specimen was taken by cotton swab: the first time was right after admission to hospital and the second time was at 2h fasting on the morning of the second day after admission. The total number of strains detected out, the positive rate of strains, the return rate of specimen, and the accuracy rate were compared between the two groups. Results The total detection number and the positive rate of strains in the sputum specimens taken at different time points were higher in observation group than in control group, and the total detection number and positive rate on getting up were higher than at random time points in both groups, the difference being statistically significant(P<0.05); the return rate at different time points was significantly lower in observation group than in control group, and the rate on getting up was lower than at random time points in both groups(P<0.05); the accuracy rate was higher in observation group than in control group at different time points, and it was higher on getting up than at random time points in both groups(P<0.05).

Key words: Bronchopneumonia, Sputum culture, Specimen collection, Collection time, Collection method, Specimen quality, Precision, Children