不同时机俞募穴速刺对脑损伤大鼠脑组织白细胞介素6和肿瘤坏死因子α表达的影响

doi:10.3969/j.issn.1674-3865.2018.05.001

中国中西医结合儿科学 ›› 2018, Vol. 10 ›› Issue (5): 369-372.doi: 10.3969/j.issn.1674-3865.2018.05.001

• 康复研究专栏 • 下一篇

不同时机俞募穴速刺对脑损伤大鼠脑组织白细胞介素6和肿瘤坏死因子α表达的影响

郭素真，马丙祥，李瑞星，李华伟，陈恬恬，张晰，叶玉香

450003 郑州，河南中医药大学第一附属医院儿科（郭素真，马丙祥，李华伟，陈恬恬，张晰，叶玉香）；450003 郑州，河南中医药大学第一临床医学院儿科（李瑞星）

出版日期:2018-10-25 发布日期:2018-12-03
通讯作者: 李瑞星，E-mail：lrixing1985@163.com
作者简介:郭素真（1980-），女，医学硕士，主治医师。研究方向：针灸治疗小儿神经系统疾病
基金资助:
河南省中医药科学研究专项课题（2014ZY02004）

Effect of rapid acupuncture in Back-shu and Front-mu acupoints at different time points on the expression of IL-6 and TNF-α in rats with brain injury

GUO Suzhen，MA Bingxiang，LI Ruixing，LI Huawei ,CHEN Tiantian，ZHANG Xi，YE Yuxiang

The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450003，China

Online:2018-10-25 Published:2018-12-03

摘要/Abstract

摘要：
目的
研究不同时间点及疗程俞募穴速刺对脑损伤大鼠脑组织白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）表达的影响，探讨俞募穴速刺对神经元的保护作用及疗效机制，为临床治疗提供实验依据。
方法
选择SPF级成熟SD大鼠雄性15只，雌性30只，适应性饲养后合笼，得孕鼠20只，将孕鼠随机分为脂多糖组（14只）和生理盐水组（6只）。对孕鼠采用腹腔注射脂多糖造成仔鼠脑损伤模型，将模型仔鼠随机分为早短期针刺组、晚短期针刺组、长期针刺组、模型对照组，另随机取正常仔鼠为空白对照组，各组10只。各针刺组取俞募穴，以速刺手法进行干预，每日1次，早短期针刺组从生后第1天开始干预，连续针刺7 d；晚短期针刺组从生后第15天开始干预，连续针刺7 d；长期针刺组从生后第1天开始干预，连续针刺21 d；模型对照组和空白对照组不给予针刺干预，各组大鼠均于21日龄取材检测脑组织IL-6和TNF-α。
结果
各针刺组下调脑组织中IL-6和TNF-α的表达，与模型对照组比较差异有统计学意义（P<0.05）；长期针刺组IL-6和TNF-α表达的下调优于短期针刺组，差异有统计学意义（P<0.05）；早短期针刺组IL-6和TNF-α表达的下调优于晚短期针刺组，差异有统计学意义（P<0.05）。
结论
俞募穴速刺可保护宫内感染/炎症所致早产脑损伤仔鼠的脑组织，建议予以早期、长期应用该疗法。

关键词: 脑损伤, 俞募穴, 时效, 白细胞介素6, 肿瘤坏死因子α, 大鼠

Abstract:
Objective
To study the effect of rapid acupuncture in Back-Shu and Front-mu acupoints at different time points and the courses on the expression of IL-6 and TNF-α in rats with brain injury, and to explore its protective effect on neurons and the therapeutic mechanism, in order to provide evidence for clinical treatment.
Methods
A total of 15 male and 30 female mature SD rats of SPF grade were chosen, and were placed in one cage after adaptive feeding, which resulted in 20 pregnant rats. These pregnant rats were randomly divided into two groups: the lipopolysaccharide group(14 rats) and normal saline group(6 rats).The brain injury model of newborn rat was established by abdominal injection of lipopolysaccharide in the pregnant rats, and these model rats were randomly divided into four groups: early and short-term acupuncture group(group A),late short-term acupuncture group(B),long-term acupuncture group(C) and model control group(D);the blank control group consisted of normal newborn rats; there were 10 rats in each group.Rapid acupuncture in the Back-shu and Front-mu acupoints was performed in the acupuncture groups, once a day; in group A it was started on the first day of birth and continued for 7 days in a row; in group B it was started on the 15th day of birth and continued for consecutively 7 days; in group C it was started on the first day of birth and continued for consecutively 21 days; the acupuncture was not performed in model control group and blank control group. On the 21st day of acupuncture, the brain tissues were taken for detection of IL-6 and TNF-α.
Results
The expression of IL-6 and TNF-α in brain tissues of the acupuncture groups was down-regulated, and there was statistical difference when compared with model control group (P<0.05).The down-regulation of IL-6 and TNF-α was better in group C than in group A and B(P<0.05),and it was better in group A than in group B(P<0.05).
Conclusion
Rapid acupuncture in Backshu and Frontmu acupoints can protect the brain tissues of premature newborn rats with brain injury due to intra-uterine infection/inflammation, so it is recommended to be sued in the early stage and for a long term.

Key words: Brain injury, Back-shu and Front-mu acupoints, Effectiveness in a given period of time, IL-6, TNF-α, Rat

郭素真，马丙祥，李瑞星，李华伟，陈恬恬，张晰，叶玉香. 不同时机俞募穴速刺对脑损伤大鼠脑组织白细胞介素6和肿瘤坏死因子α表达的影响[J]. 中国中西医结合儿科学, 2018, 10(5): 369-372.

GUO Suzhen，MA Bingxiang，LI Ruixing，LI Huawei,CHEN Tiantian，ZHANG Xi，YE Yuxiang. Effect of rapid acupuncture in Back-shu and Front-mu acupoints at different time points on the expression of IL-6 and TNF-α in rats with brain injury[J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2018, 10(5): 369-372.

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不同时机俞募穴速刺对脑损伤大鼠脑组织白细胞介素6和肿瘤坏死因子α表达的影响

Effect of rapid acupuncture in Back-shu and Front-mu acupoints at different time points on the expression of IL-6 and TNF-α in rats with brain injury

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