ISSN 1674-3865  CN 21-1569/R
主管:国家卫生健康委员会
主办:中国医师协会
   辽宁省基础医学研究所
   辽宁中医药大学附属医院

中国中西医结合儿科学 ›› 2019, Vol. 11 ›› Issue (2): 112-116.doi: 10.3969/j.issn.1674-3865.2019.02.006

• 实验论著 • 上一篇    下一篇

高效液相色谱法同时测定小儿补肾固表颗粒中6种成分的含量

杨辉,潘东明,戚欢阳,黄亦琦,罗友华,张青玲,薛征,虞坚尔,褚克丹   

  1. 350122 福建 福州,福建中医药大学药学院(杨辉,潘东明,张青玲,褚克丹);361008福建 厦门,厦门市医药研究所厦门市天然药物研究与开发重点实验室(杨辉,潘东明,戚欢阳,黄亦琦,罗友华,张青玲);200071 上海,上海中医药大学附属市中医医院儿科(薛征,虞坚尔)
  • 出版日期:2019-04-25 发布日期:2019-05-08
  • 通讯作者: 褚克丹,E-mail:13950280590@163.com
  • 作者简介:杨辉(1975-),女,副主任药师。研究方向:中药制剂与质量控制研究
  • 基金资助:
    厦门市科技惠民项目(3502Z20164085);上海市科委科研项目(15401902800)

Simultaneous determination of six components in Xiaoer Bushen Gubiao granule by HPLC

YANG Hui,PAN Dongming,QI Huanyang,HUANG Yiqi, LUO Youhua, ZHANG Qingling,XUE Zheng, YU Jianer,CHU Kedan   

  1. College of Pharmacy,Fujian University of Traditional Chinese Medicine, Fuzhou 350122,China
  • Online:2019-04-25 Published:2019-05-08

摘要:
目的建立高效液相色谱法同时测定小儿补肾固表颗粒中6种成分(升麻素苷、毛蕊异黄酮葡萄糖苷、5-O-甲基维斯阿米醇苷、黄芩苷、补骨脂素和异补骨脂素)含量的方法。
方法采用C18色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.2%甲酸水溶液,梯度洗脱,流速1.0 mL/min,柱温30 ℃,进样量10 μL,DAD紫外检测器,检测波长254 nm和280nm。
结果6种成分的分离度良好且在各自浓度范围内与峰面积呈良好线性关系(r≥0.999 0),升麻素苷、毛蕊异黄酮葡萄糖苷、5-O-甲基维斯阿米醇苷、黄芩苷、补骨脂素和异补骨脂素的平均加样回收率分别为99.89%、101.86%、103.22%、99.59%、98.37%、98.12%,RSD分别为3.84%、2.80%、3.12%、4.29%、4.53%、4.87%。
结论高效液相色谱法准确、简便、重复性好且灵敏度高,可用于小儿补肾固表颗粒中多成分的质量控制研究。

关键词: 小儿补肾固表颗粒, 升麻素苷, 毛蕊异黄酮葡萄糖苷, 5-O-甲基维斯阿米醇苷, 黄芩苷, 补骨脂素, 异补骨脂素, 高效液相色谱

Abstract:
Objective
To establish a method for the simultaneous determination of six components (prim-O-glucosylcimifugin,calycosin-7-glucoside, 5-O-methylvisammioside, baicalin, psoralen and angelicin) in Xiaoer Bushen Gubiao granule by HPLC.
Methods
The chromatographic conditions were determined by high performance liquid chromatography with C18 column (250 mm×4.6 mm,5 μm),mobile phase being acetonitrile-0.2% formic acid aqueous for solution gradient elution; flow rate was 1.0 mL/min,and column temperature was 30 ℃, injection volume 10 μL; DAD detector was used with the detection wavelength being 254 nm and 280 nm.
Results
The six components were well separated and showed a good linear relationship with peak area (r≥0.999 0) within their respective concentration ranges. The average sample recovery rates of prim-O-glucosylcimifugin,calycosin-7-glucoside, 5-O-methylvisammioside, baicalin, psoralen and angelicin were 99.89%,101.86%,103.22%,99.59%,98.37%,98.12%,and RSD was 3.84%,2.80%,3.12%,4.29%,4.53% and 4.87%, respectively.
Conclusion
The method is accurate, simple, reproducible and sensitive. It can be used for the quality control of multi-components in Xiaoer Bushen Gubiao granule.

Key words: Xiaoer Bushen Gubiao granules, Prim-O-glucosylcimifugin, Calycosin-7-glucoside, 5-O-methylvisammioside, Baicalin, Psoralen, Angelicin, HPLC