ISSN 1674-3865  CN 21-1569/R
主管:国家卫生健康委员会
主办:中国医师协会
   辽宁省基础医学研究所
   辽宁中医药大学附属医院

中国中西医结合儿科学 ›› 2017, Vol. 9 ›› Issue (2): 93-96.doi: 10.3969/j.issn.1674-3865.2017.02.001

• 实验论著 •    下一篇

清肺通络敷胸膏对流感病毒肺炎大鼠肺组织ERK/NF-κB p65蛋白表达的影响

刘双,王雪峰,郝欧美   

  1. 110032 沈阳,辽宁中医药大学2014级中医儿科学专业研究生(刘双);辽宁中医药大学附属医院儿科(王雪峰,郝欧美)
  • 出版日期:2017-04-25 发布日期:2017-12-18
  • 通讯作者: 王雪峰,E-mail:lnzywxf@163.com
  • 作者简介:刘双(1989-),女,辽宁中医药大学2014级硕士研究生在读。研究方向:病毒性疾病的中医药治疗
  • 基金资助:
    国家自然科学基金项目(81273800)

Effect of Qingfei Tongluo ointment on the expression of ERK/NF-κB p65 protein in the lung tissues of rats with influenza virus pneumonia

LIU Shuang,WANG Xuefeng,HAO Oumei   

  1. Liaoning University of Traditional Chinese Medicine, Shenyang 110032,China
  • Online:2017-04-25 Published:2017-12-18

摘要:
目的:探讨清肺通络膏对流感病毒诱导的肺炎大鼠肺组织中ERK/NF-κB p65信号通路的调控机制。

方法:将30只Wister大鼠随机分为正常组、模型组、清肺通络敷胸膏组,每组10只。除正常组外,采用鼻腔接种流感病FM1株诱导Wister幼龄大鼠肺炎,在感染后各治疗组采用清肺通络膏贴敷于大鼠背部肺脏投影区治疗。观察各组大鼠的一般状态(饮食量、进水量、毛发、活动量、精神状态、呼吸频率);光镜下观察肺组织形态学;采用免疫组织化学法检测各组大鼠肺组织磷酸化细胞外调节蛋白激酶(p-ERK),核转录因子(NF-κB p65)的表达水平。
结果:与正常组相比,模型组大鼠肺组织中p-ERK,NF-κB p65蛋白表达显著增高,差异有统计学意义(P<0.05),清肺通络膏组p-ERK、NF-κB p65蛋白的表达较模型组明显降低,差异有统计学意义(P<0.05)。
结论:清肺通络膏通过抑制ERK磷酸化,使NF-κB p65表达降低,从而减轻了肺组织的病理损伤,达到治疗目的。

关键词: 肺炎, 流感病毒, 清肺通络膏, 细胞外调节蛋白激酶, 核转录因子p65, 大鼠

Abstract:
Objective:To investigate the mechanism of Qingfei Tongluo ointment in regulating the EPK/NF-κB p65 signal pathway in the lung tissues of rats with influenza virus pneumonia.

Methods:Thirty Wister rats were randomly divided into 3 groups: normal group(A), model group(B) and Qingfei Tongluo group(C), 10 rats in each group. Influenza virus FM1 strain was inoculated through nose to induce pneumonia in young Wister rats, in group B and C. After infection, Qingfei Tongluo ointment was applied to the projected area of lungs on the back of rats for treatment. The general state of all the rats were observed, including the amount of food and water taken, hair,exercise, mental state and breathing frequency. The morphology of lung tissues was observed under light microscope. The expression of NF-κB p65 and pERK was detected by immunohistochemical assay.
Results:Compared with group A, the expression of p-ERK and NF-κB p65 protein in the lung tissues of rats in group B was significantly increased, and the difference was statistical(P<0.05);the expression of p-ERK and NF-κB p65 in group C was significantly reduced compared with group B, and there was statistical difference(P<0.05).
Conclusion:Qingfei Tongluo ointment can reduce the expression NF-κB p65 through inhibiting the phosphorylation of ERK, thus alleviating the pathological damage of lung tissues and achieving the treatment effect.

Key words: Pneumonia, Influenza virus, Qingfei Tongluo ointment, p-ERK, NF-κB p65, Rat