To observe the effect of Yiqi Guben capsule on the levels of TNFα、IL1β in serum and alveolar lavage fluid of asthmatic mice, and to explore the mechanism of Yiqi Guben capsule in inhibiting airway remodeling in asthma.
Sixty BALB/c mice were randomly divided into normal control group(n＝10),model group(n＝14),Yiqi Guben capsules group(n＝12),dexamethasone tablets group(n＝12) and Tongkang tablets group(n＝12).Yiqi Guben capsules(55 g/L),dexamethasone tablets(0.005 g/L)and Tongkang tablets(44 g/L) were given by intragastric administration to each drug group, and normal control group and model group were given equal volume of pure water by intragastric administration, once a day for 30 consecutive days. Asthma mice models were established by sensitization with ovalbumin(OVA) according to the literature. Asthma models were challenged once a day for 10 consecutive days beginning on the 21st day after administration. The drug groups were given the tested drug 1 hour before the provocation. The content of TNFα and IL1β in serum and alveolar lavage fluid were determined by enzymelinked immunosorbent assay (ELISA) after 30 days.
Compared with normal control group, Serum levels of TNFα in model group statistically were significantly increased(P＜0.01). Compare with asthma model group, serum levels of TNFα in Yiqi Guben capsule group and Tongkang tablet group and dexamethasone tablets group were decreased significantly (P＜0.05),serum levels of IL1β was no statistical significance(P＞0.05). Compare with normal control group,bronchoalveolar lavage fluid levels of TNFα and IL1β in model group were significantly increased(P＜0.01).Compare with asthma model group,bronchoalveolar lavage fluid levels of TNFα and IL1β in Yiqi Guben capsule group and Tongkang tablet group and dexamethasone tablets were decreased significantly (P＜0.05).
ConclusionYiqi Guben capsule can effectively reduce the levels of TNFα in serum and TNFα，IL1β in bronchoalveolar lavage fluid of asthmatic mice. It is suggested that it may treat asthma by inhibiting the activity of NFκB signal pathway by reducing the levels of TNFα，IL1β in mice.
孔一卜，孙丽平，刘璐. 益气固本胶囊调控哮喘小鼠核因子κB信号通路肿瘤坏死因子α和白细胞介素1β含量的实验研究[J]. 中国中西医结合儿科学, 2018, 10(5): 409-412.
KONG Yibu, SUN Liping，LIU Lu. . Experimental study on the effect of Yiqi Guben capsule in regulating the content of TNFα、IL1β in NFκB signaling pathway of asthma mice#br#. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2018, 10(5): 409-412.