ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2019, Vol. 11 ›› Issue (1): 12-14.doi: 10.3969/j.issn.1674-3865.2019.01.004

• 实验论著 • 上一篇    下一篇

人胎盘来源间充质干细胞对T淋巴细胞分泌白细胞介素-4和γ干扰素影响的实验研究

张莉，吴素琴，孙晓   

  1. 110014 沈阳，沈阳市妇婴医院辅助生殖中心（张莉，孙晓）；110121 沈阳，辽宁省基础医学研究所免疫研究室（吴素琴）
  • 出版日期:2019-02-25 发布日期:2019-07-11
  • 通讯作者: 孙晓，E-mail：13700044048@163.com
  • 作者简介:张莉（1980-），女，医学博士，副主任医师。研究方向：生殖医学
  • 基金资助:
    辽宁省科学技术基金项目（20170540838）

Experimental study on the influence of placenta-derived mesenchymal stem cells on the secretion of IL-4 and IFN-γ in T lymphocytes

ZHANG Li，WU Suqin,SUN Xiao   

  1. Shenyang Women's and Children's Hospital，Shenyang 110014，China
  • Online:2019-02-25 Published:2019-07-11

PDF (PC)

94

摘要：
目的
观察人胎盘来源间充质干细胞对活化的T淋巴细胞分泌白细胞介素4（IL-4）、γ干扰素（IFN-γ）能力的影响，推测人胎盘来源间充质干细胞应用于临床治疗多种疾病的可能性。
方法分离两个健康人来源的T淋巴细胞，制备混合淋巴细胞培养（MLC）体系。再获取人胎盘来源间充质干细胞，将人胎盘来源间充质干细胞与MLC体系混合共培养。共分为4组，分别是同源T细胞Ⅰ组、同源T细胞Ⅱ组、共培养组、阳性对照组，每组接种10孔。培养72 h。采用ELISA法检测各组细胞培养上清液中IL-4、IFN-γ的含量。
结果同源T细胞Ⅰ、Ⅱ组组间细胞培养上清液中IL-4和IFN-γ比较差异无统计学意义（P>0.05）；与同源T细胞Ⅰ、Ⅱ组比较，共培养组和阳性对照组细胞培养上清液中IL-4和IFNγ浓度显著升高，差异有统计学意义（P<0.01）；与共培养组比较，阳性对照组细胞培养上清液中IL-4浓度显著升高，IFN-γ浓度显著降低，差异有统计学意义（P<0.01）。
结论人胎盘来源间充质干细胞可抑制活化的T淋巴细胞分泌IL-4的能力、促进IFN-γ的能力，胎盘间充质干细胞可能具有促进Th0向Th1细胞分化的作用。

关键词: 人胎盘来源间充质干细胞, MLC体系, 共培养, 白细胞介素-4, γ干扰素

Abstract:
Objective
To observe the influence of placenta-derived mesenchymal stem cells on the secretion of IL-4 and IFN-γ in activated T lymphocytes, and to predict the possibility of applying the placenta-derived mesenchymal stem cells in the clinical treatment of various diseases.
Methods
T lymphocytes were isolated from two healthy peoples to make MLC system. The placenta-derived mesenchymal stem cells were obtained, which were co-cultured with MLC system. They were divided into four groups: homologous T cells group Ⅰ, homologous T cell group Ⅱ, co-culture group and positive control group, and each group was inoculated with 10 holes. The culture lasted for 72h. ELISA was used to detect the content of IL-4 and IFN-γ in the cell culture supernatant of each group.
Results
There was no significant difference in IL-4 and IFN-γ in the supernatant between homologous groupⅠand group Ⅱ(P>0.05). compared with group Ⅰ and group Ⅱ, the concentration of IL-4 and IFN-γ was significantly increased in co-culture group and positive control group(P<0.01). Compared with co-culture group, the IL-4 concentration in positive control group was significantly increased, while IFN-γ concentration was significantly decreased, and the difference was statistically significant(P<0.01).
Conclusion
The placenta-derived mesenchymal stem cells can inhibit the ability of activated T lymphocytes to secrete IL-4 and to promote IFN-γ. They may have the ability to promote Th0 to differentiate into Th1.

Key words: Human placenta-derived mesenchymal stem cells, MLC system, Co-culture, IL-4, IFN-γ