ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2018, Vol. 10 ›› Issue (6): 461-465.doi: 10.3969/j.issn.1674-3865.2018.06.001

• 专栏 •    下一篇

基于“肺肠理论”和胆汁酸代谢网络探讨定喘汤对呼吸道合胞病毒感染大鼠肠道菌群调节的研究

崔振泽，徐超，迟磊   

  1. 116000 辽宁 大连，大连市儿童医院呼吸科
  • 出版日期:2018-12-25 发布日期:2019-03-25
  • 通讯作者: 崔振泽，E-mail：cuizhenze@126.com
  • 作者简介:崔振泽（1964-），男，医学博士，教授、主任医师，硕士研究生导师。研究方向：中西医结合防治小儿呼吸系统疾病
  • 基金资助:
    国家自然科学基金项目（81473726）

Study on Dingchuan decoction based on "Lung-Intestine Theory" and bile acid metabolism network in the regulation of intestinal flora in rats with RSV infection

CUI Zhenze, XU Chao, CHI Lei   

  1. Dalian Children's Hospital, Dalian 116000, China
  • Online:2018-12-25 Published:2019-03-25

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摘要：
目的
通过观察呼吸道合胞病毒（RSV）感染大鼠后血清中胆汁酸代谢物质的变化来间接探讨其导致的肠道菌群的改变，并基于“肺肠理论”进一步阐明定喘汤对RSV感染后胆汁酸代谢及肠道菌群微生态的调节机制。
方法
将Wistar大鼠随机分为正常组、RSV模型组和定喘汤组，除正常组外，其余各组用RSV Long株病毒液进行滴鼻接种造模。通过观察各组大鼠一般状态及病理改变指标进行评估，并应用液相色谱-质谱联用技术（LC-MS）进行相关代谢产物的检测。
结果
（1）定喘汤可不同程度的调节RSV感染后大鼠的一般状态、降低RSV感染后的肺指数及改善病理变化；（2）与正常组大鼠比较，RSV感染组大鼠血清中柠檬酸(CA)、牛磺胆酸（TCA）、牛磺熊脱氧胆酸（TUDCA）、鹅脱氧胆酸(CDCA）、7-酮基脱氧胆酸（7-ketodeoxycholic acid）及苯乙酸（Phenylacetic acid）水平显著升高，甘氨鹅脱氧胆酸（GCDCA）显著下降，差异有统计学意义（P<0.05）；与RSV模型组比较，定喘汤组大鼠血清中柠檬酸(CA)、牛磺胆酸（TCA）、牛磺熊脱氧胆酸（TUDCA）、鹅脱氧胆酸(CDCA）、甘氨鹅脱氧胆酸（GCDCA）、7-酮基脱氧胆酸（7-ketodeoxycholic acid）及苯乙酸（Phenylacetic acid）均有所回调，差异有统计学意义（P<0.05）。
结论
定喘汤能有效调节RSV感染后大鼠血清中胆汁酸代谢的失衡，进而维持了肠道菌群微生态的稳定性。

关键词: 呼吸道合胞病毒, 肺肠理论, 定喘汤, 胆汁酸代谢, 肠道菌群, 大鼠

Abstract:
Objective
By observing the changes of bile acid metabolites in serum of RSV-infected rats, we observed indirectly the changes of intestinal flora caused by RSV. Based on the "Lung-Intestine Theory", the regulation mechanism of Dingchuan decoction on bile acid metabolism and intestinal microflora after RSV infection was further elucidated.
Methods
Wistar rats were randomly divided into normal control group, RSV infection model group and Dingchuan decoction group. Except the normal control group, the other groups were intranasally inoculated with RSV Long strain virus solution. The general state and pathological changes of each group of rats were evaluated, and the related metabolites were detected by liquid chromatography-mass spectrometry(LC-MS).
Results
(1)Dingchuan decoction could regulate the general state of rats after RSV infection, reduce the lung index after RSV infection and improve pathological changes. (2)Compared with normal group, the levels of serum CA(citrate), TCA(taurocholic acid), TUDCA(tauroursodeoxycholic acid), CDCA(chenodeoxycholic acid), 7-ketodeoxycholic acid and phenylacetic acid in RSVinfected rats were significantly increased, while GCDCA(glycine chenodeoxycholic acid) was significantly decreased, and the difference was statistically significant（P＜0.05）；compared with the RSV model group, the serum levels of CA(citric acid), TCA(taurocholic acid), TUDCA(tauroursodeoxycholic acid), CDCA(chenodeoxycholic acid), GCDCA(glycine chenodeoxycholic acid), 7-ketodeoxycholic acid and phenylacetic acid of Dingchun decoction group were all downregulated, the difference being statistically significant.
ConclusionDingchuan decoction can effectively regulate the imbalance of bile acid metabolism in serum of rats after RSV infection, and thus maintain the stability of intestinal microflora.

Key words: Respiratory syncytial virus, Lung-intestine Theory, Dingchuan decoction, Bile acid metabolism, Intestinal flora, Rat