ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2017, Vol. 9 ›› Issue (5): 369-371.doi: 10.3969/j.issn.1674-3865.2017.05.001

• 实验论著 •    下一篇

定喘汤对呼吸道合胞病毒感染幼龄大鼠肺泡灌洗液中炎症细胞的影响

崔振泽，黄燕，徐超   

  1. 116000 辽宁 大连，大连市儿童医院呼吸科
  • 出版日期:2017-10-25 发布日期:2017-12-18
  • 通讯作者: 黄燕，E-mail：13304280868@126.com
  • 作者简介:崔振泽（1964-），男，医学博士，教授、主任医师，硕士研究生导师。研究方向：中西医结合防治小儿呼吸系统疾病 通讯作者：黄燕，E-mail：13304280868@126.com
  • 基金资助:
    国家自然科学基金项目（81473726）|辽宁省自然科学基金项目（2014020173）

Effect of Dingchuan decoction on the inflammatory cells in BALF of young rats infected with RSV

CUI Zhenze，HUANG Yan，XU Chao   

  1.  Dalian Children's Hospital，Dalian 116000，China
  • Online:2017-10-25 Published:2017-12-18

PDF (PC)

127

摘要：
目的
观察呼吸道合胞病毒（RSV）感染幼龄大鼠后肺泡灌洗液（BALF）中细胞总数及分类的变化及定喘汤对其的影响。
方法
将27只Wistar大鼠随机分为正常组、RSV模型组、定喘汤组，每组9只。除正常组外，余各组用RSV（Long珠）病毒予大鼠滴鼻造模，造模后正常组和RSV模型组均以等体积的生理盐水灌胃，定喘汤组给予定喘汤灌胃。采用苏木精-伊红染色观察各组大鼠肺组织炎症变化情况|采用细胞涂片瑞氏-吉姆萨染色方法检测各组大鼠BALF中细胞分类计数。
结果
病理结果显示，RSV模型组大鼠肺组织炎症明显，炎症细胞增多，定喘汤干预后肺组织炎症有所好转。RSV模型组细胞总数及各分类指标均高于正常组，差异有统计学意义（P<0.05）|定喘汤组细胞总数及各分类指标均低于RSV模型组，差异有统计学意义（P<0.05）。
结论
定喘汤干预RSV感染大鼠的作用机制可能与抑制大鼠BALF中的炎症细胞有关。

关键词: 呼吸道合胞病毒感染, 定喘汤, 细胞计数, 大鼠

Abstract:
Objective
To observe the changes of the total number and classification of BALF in young rats infected with RSV as well as the effect of Dingchuan decoction on it.
Methods
Twenty-seven Wistar rats were randomly divided into normal group, RSV model group and Dingchuan decoction group, 9 rats in each group. Except for the normal group, the rats in the other two groups were given RSV(Long bead) virus for nose dripping to get modeling, and after modeling the rats in normal group and model group were given gavage with equal volume of physiological saline. The Dingchuan decoction group was gavaged with Dingchuan decoction. HE staining method was used to observe the change of inflammation in lung tissues of rats; the cell smear-Ruish-Giemsa staining was used to determine the cell classification count in BALF of rats in each group.
Results
Pathology displayed: compared with the normal group, the inflammation of lung tissue in RSV model group was obvious and inflammatory cells increased, and after Dingchuan decoction intervention lung tissue inflammation was improved; compared with the normal group, the total cell count and the indexes of RSV model group were higher, and the difference was statistically significant(P<0.05). Compared with the model group, the total cell count and indexes of Dingchuan decoction group were significantly decreased, and the difference was statistically significant(P<0.05).
Conclusion
The mechanism of Dingchuan decoction in RSV infection in rats may be related to the inhibition of inflammatory cells in rat BALF.

Key words: RSV, Dingchuan decoction, Cell count, Rat