ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2017, Vol. 9 ›› Issue (5): 386-389.doi: 10.3969/j.issn.1674-3865.2017.05.006

• 临床研究 • 上一篇    下一篇

川崎病患儿急性期单核细胞S100 A8/A9蛋白表达及其与冠状动脉损害的关系

胡静，钱炜，李红，李琪   

  1. 214000 江苏 无锡，无锡市儿童医院心内科
  • 出版日期:2017-10-25 发布日期:2017-12-18
  • 通讯作者: 钱炜，E-mail：qianyizhou@163.com
  • 作者简介:胡静（1981-），女，医学硕士，主治医师。研究方向：小儿心血管系统疾病的诊治

Expression of S100 A8/A9 protein in children with acute Kawasaki disease and its relationship with coronary artery lesions

HU Jing，QIAN Wei，LI Hong，LI Qi   

  1. Wuxi Children's Hospital，Wuxi 214000，China
  • Online:2017-10-25 Published:2017-12-18

PDF (PC)

139

摘要：
目的
探讨单核细胞及炎性蛋白S100 A8/A9在川崎病的发展及冠状动脉损伤的发生过程中可能起到的作用。
方法
选择2010年1月至2012年12月无锡市儿童医院心内科收治住院的川崎病患儿46例为研究对象，按冠状动脉有无损害分为冠状动脉损害组20例和无冠状动脉损害组26例。同期选取本院行腹股沟疝气择期手术患儿40例为对照组。收集外周静脉血，采用淋巴细胞分层液密度梯度离心法分离单个核细胞，提取总RNA，用RT-PCR法检测S100 A8/A9 mRNA的表达，ELISA法检测血浆S100A8/A9蛋白二聚体的水平。
结果
川崎病患儿急性期未予静脉注射用免疫球蛋白治疗时，冠状动脉损害组和无冠状动脉损害组血浆S100 A8/A9蛋白二聚体水平均显著高于对照组，差异有统计学意义（P<0.05）|冠状动脉损害组和无冠状动脉损害组患儿在使用静脉注射用免疫球蛋白前单核细胞中S100 A8/A9 mRNA表达均显著高于对照组，差异有统计学意义（P<0.05）。冠状动脉损害组血浆S100 A8/A9蛋白二聚体水平及S100 A8/A9 mRNA的表达高于无冠状动脉损害组，差异有统计学意义（P<0.05）。
结论
川崎病急性期单核细胞S100 A8/A9 mRNA表达及二聚体水平在使用静脉注射用免疫球蛋白前均显著高于对照组，提示S100 A8/A9可能参与了川崎病的发病机制。

关键词: 川崎病, 单核细胞, S100 A8, S100 A9, 儿童

Abstract:
Objective
To investigate the role of S100 A8/A9 protein in the development of Kawasaki disease and the occurrence of coronary artery lesions.
Methods
A total of 46 children with Kawasaki disease were included as the research subjects, who were treated in Wuxi Children's Hospital from Jan. 2010 to Dec. 2012,and they were divided into two groups based on whether there was coronary artery lesion or not: 20 in the with-lesion group(A) and 26 in the without-lesion group(B). Another 40 children receiving operation for inguinal hernia were chosen as the control group. Peripheral venous blood was collected; the monocytes were separated by stratified fluid density gradient centrifugation of lymphocytes; RNA was extracted; expression of S100 A8/A9 was detected by RT-PCR; the dimer level of plasma S100 A8/A9 was determined by ELISA.
Results
Before the venous injection of immune globulin for children with acute Kawasaki disease, the dimer level of S100 A8/A9 protein in group A and group B was significantly higher than that in control group, the difference being statistically significant(P<0.05),while after the injection, the expression of S100 A8/A9 mRNA was significantly higher than that in control group, and there was statistical difference(P<0.05);the dimer level of S100 A8/A9 protein and S100 A8/A9 mRNA expression in group A were higher than those in group B, with statistical difference(P<0.05).
Conclusion
Before venous injection of immune globulin, the S100 A8/A9 mRNA expression and dimer level is significantly higher than the control group, which suggests that S100 A8/A9 may be involved in the pathogenesis of Kawasaki disease.

Key words: Kawasaki disease, Monocytes, S100 A8, S100 A9, Child