ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2016, Vol. 8 ›› Issue (3): 257-260.doi: 10.3969/j.issn.1674-3865.2016.03.003

• 实验论著 • 上一篇    下一篇

缺氧缺血致脑白质损伤的新生大鼠脑中ephrinB2和EphB4表达的研究

房贵巍，张丽华，许洪伟，孙颖   

  1. 154000 黑龙江 佳木斯，佳木斯大学康复医学院研究生处(房贵巍)|154002 黑龙江 佳木斯，佳木斯大学附属第三医院脑瘫康复二科(张丽华，许洪伟，孙颖)|佳木斯大学儿童神经康复实验室(张丽华，许洪伟，孙颖)
  • 出版日期:2016-06-25 发布日期:2018-11-19
  • 通讯作者: 张丽华，E-mail：zhanglihua59597@qq.com
  • 作者简介:房贵巍(1990-)，女，佳木斯大学康复医学院2013级硕士研究生在读。研究方向：小儿脑性瘫痪的综合康复治疗

Expression of ephrinB2 and EphB4 in a neonatal rat model of periventricular white matter damage resulting from hypoxia-ischemia

FANG Guiwei,ZHANG Lihua,XU Hongwei,SUN Ying   

  1. The Third Affiliated Hospital of Jiamusi University，Children Neural Rehabilitation Laboratory of Jiamusi University，Jiamusi 154000，China.
  • Online:2016-06-25 Published:2018-11-19

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摘要：
目的: 评估缺氧缺血诱导的脑白质损伤大鼠模型脑组织中的微血管密度和ephrinB2及其受体EphB4表达的水平。
方法: 将新生3日龄Wistar大鼠60只随机分为实验组和假手术组各30只。实验组行右侧颈总动脉永久性结扎后给予氮氧混合气体(94% N2+6% O2)4 h|假手术组只分离右侧颈总动脉但不结扎并常规给氧。4 d后分别测定第7日龄大鼠脑组织中微血管密度以及ephrinB2和EphB4表达的水平。
结果: 实验组大鼠脑组织中的微血管密度、ephrinB2和EphB4的水平高于假手术组|假手术组大鼠脑组织中表达的ephrinB2和EphB4的百分比相近，实验组大鼠脑组织中ephrinB2的表达高于EphB4。
结论: 缺氧缺血诱导的脑白质损伤后有新生血管生成，并且动脉多于静脉。

关键词: 脑白质损伤, 缺氧缺血, 微血管密度, ephrinB2, EphB4, 大鼠

Abstract:
Objective: To evaluate microvessel density (MVD) and the expression of ephrinB2 and its receptor EphB4 in a hypoxia-ischemia induced PWMD rat model.
Methods: Postnatal day 3 Wister rats(n=60) were randomly divided into hypoxia ischemia group(group HI),sham operation group(control group),with 30 rats in each group.The HI group underwent permanent ligation of the right common carotid artery then they were exposed to hypoxia (94% N2+6% O2) for 4 hours.For the control group rats,the same surgery was performed except for ligation and exposure to hypoxia.MVD and levels of ephrinB2 and EphB4 were determined at postnatal day 7.
Results: Compared with control group,MVD,ephrinB2 and EphB4 levels were higher in the brains of hypoxic-ischemic rats.Similar percentages of vessels expressed ephrinB2 and EphB4 in control group,but expression of ephrinB2 was greater in brains injured by hypoxia-ischemia.
Conclusion: Following hypoxic-ischemic injury to the rat brain,there were angiogenesis and more arterioles than venules are acquired.

Key words: Periventricular white matter damage, Hypoxia-ischemia, Microvessel density, EphrinB2, EphB4, Rat