ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2016, Vol. 8 ›› Issue (3): 261-264.doi: 10.3969/j.issn.1674-3865.2016.03.004

• 实验论著 • 上一篇    下一篇

柴芍平痫汤对癫痫幼龄大鼠脑内γ-氨基丁酸含量的影响

魏冲，张葆青   

  1. 250014 济南，山东中医药大学(魏冲)|250011济南，山东中医药大学附属医院儿科(张葆青)
  • 出版日期:2016-06-25 发布日期:2018-11-19
  • 通讯作者: 张葆青，E-mail：459356800@qq.com
  • 作者简介:魏冲(1989-)，男，山东中医药大学2008级七年制本硕连读生。研究方向：小儿呼吸系统、神经系统疾病的研究

Effect of Chaishao Pingxian(epilepsy-relieving) decoction on brain GABA content of young rats with epilepsy

WEI Chong,ZHANG Baoqing   

  1. Shandong University of Traditional Chinese Medicine，Jinan 250014，China.
  • Online:2016-06-25 Published:2018-11-19

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摘要：
目的: 研究柴芍平痫汤对癫痫幼龄大鼠脑内γ-氨基丁酸含量的影响，探索其作用机制。
方法: 70只SD大鼠随机分为7组，分别为中药低、中、高剂量组、西药组、中西药治疗组、模型组、正常对照组，每组10只。对SD大鼠腹腔注射戊四氮建立癫痫模型，观察行为学，以柴芍平痫汤对其进行干预治疗24 d后ELISA法检测脑组织γ-氨基丁酸(GABA)的含量。
结果: 中西药治疗组用药后24 d惊厥等级与用药前1 d惊厥等级比较差异有统计学意义(P<0.05)，其余各组无差异|中药低、中、高剂量组，西药组，中西药治疗组均比模型组脑组织内γ-氨基丁酸含量高，差异有统计学意义(P<0.01)|各治疗组及模型组γ-氨基丁酸含量与正常对照组比较差异有统计学意义(P<0.01)。
结论: 通过实验得出柴芍平痫汤对癫痫大鼠惊厥等级、脑组织内γ-氨基丁酸含量的降低均有治疗作用。

关键词: 癫痫, 戊四氮, γ-氨基丁酸, 卡马西平, 儿童

Abstract:
Objective: To study the effect of Chaishao Pingxian decoction on brain GABA content of young rats with epilepsy and to investigate its mechanism.
Methods: The rats were randomly divided into 7 groups,by number,namely,low-(A),moderate(B) and high-dose(C) group,western medicine group(D),Chinese and western medicine group(E),model group(F) and normal control group(G),with 10 rats in each group.PTZ was injected into SD rats to establish epilepsy model and the behavior was observed.Chaishao Pingxian decoction was given as intervention treatment and after 24 days ELISA was used to determine GABA content in brain tissues.
Results: There was statistical difference on convulsion grade between 24d after treatment and 1d before treatment in group E,but no difference in the other groups.Compared with group F,GABA content was higher in group A,B,C,D and E,and difference was statistical(P<0.01).When comparing GABA content of group G with that of the other six groups,there was statistical difference(P<0.01).
Conclusion: Chaishao Pingxian decoction has therapeutic effect on the epilepsy grade and the decrease of GABA content in brain tissues in tats with epilepsy.

Key words: Epilepsy, PTZ, GABA, CBZ, Rat