ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2018, Vol. 10 ›› Issue (1): 6-8.doi: 10.3969/j.issn.1674-3865.2018.01.002

• 实验论著 • 上一篇    下一篇

壮药防病香囊对气虚小鼠呼吸道sIgA及IgG分泌的影响

王力宁,张毅贤,邓普元,李洁,周志宁   

  1. 530023 南宁，广西中医药大学第一附属医院儿科
  • 出版日期:2018-02-25 发布日期:2018-03-23
  • 通讯作者: 王力宁，E-mail：wln21@126.com
  • 作者简介:王力宁（1956-），女，教授。研究方向：哮喘与肺系疾病的防治
  • 基金资助:
    国家中医药管理局国家中医临床研究基地业务建设科研专项课题(JDZX2015040）；广西名中医民族医传承工作室建设项目(桂卫中〔2013〕10号)

Effects of Anti-disease Sachet(a type of Zhuang medicine) on the secretion of respiratory sIgA and IgG in Qi-deficiency mice

WANG Lining，ZHANG Yixian，DENG Puyuan，LI Jie，ZHOU Zhining   

  1. First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine，Nanning 530023，China
  • Online:2018-02-25 Published:2018-03-23

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摘要： 目的
观察壮药防病香囊对气虚小鼠局部免疫功能的影响，探讨其预防反复呼吸道感染的部分疗效机制。
方法
选用SPF级昆明种小白鼠24只，体质量18～22 g，雌雄兼用，随机分为空白组、模型组、香模组、香囊组，每组6只。先予壮药防病香囊连续干预4周，并于第4周开始造气虚模型，造模成功后予ELISA法检测肺泡灌洗液sIgA及IgG的浓度。
结果
模型组与香模组小鼠肺泡灌洗液中sIgA比较差异无统计学意义（P>0.05）；香囊组小鼠肺泡灌洗液中sIgA显著高于空白组和模型组，差异有统计学意义（P<0.05）。4组小鼠肺泡灌洗液中IgG比较差异无统计学意义（P>0.05）。
结论
壮药防病香囊有提高呼吸道免疫功能的作用，可用于预防小儿反复呼吸道感染。

关键词: 气虚, 壮药防病香囊, sIgA, IgG, 小鼠

Abstract: Objective
To study the effect of Anti-disease Sachet(a type of Zhuang medicine) on local immune function in Qi-deficiency mice, and to explore its mechanism in prevention and treatment of recurrent respiratory tract infection.
Methods
A total of 24 SPF mice of Kunming species were chosen(male and female), whose body mass was 18-22 g. They were randomly divided into four groups: blank group, model group, incense module group and sachet group, with 6 mice in each group. They were intervened by Anti-disease Sachet for 4 weeks, and at the fourth week, Qi-deficiency model was established; after that, ELISA was used to determine the concentration of sIgA and IgG in the alveolar lavage fluid.
Results
There was no statistical difference in sIgA in alveolar fluid between model group and sachet group(P>0.05). The sIgA in alveolar fluid of mice was significantly higher in sachet group than in blank group and model group, and there was statistical difference(P<0.05). There was no statistical difference in IgG in alveolar fluid of mice among the four groups(P>0.05).
Conclusion
Anti-disease Sachet(a type of Zhuang medicine) is effective in improving respiratory immune function, so it can be used to prevent recurrent respiratory tract infection in children.

Key words: Qi-deficiency, Anti-disease Sachet(a type of zhuang medicine), sIgA, IgG, Mice