ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2017, Vol. 9 ›› Issue (6): 472-474.doi: 10.3969/j.issn.1674-3865.2017.06.003

• 实验论著 • 上一篇    下一篇

肾综颗粒对肾病综合征大鼠微炎症的影响

孙响波，于妮娜，潘月丽   

  1. 266000 山东 青岛，青岛市海慈医疗集团（青岛市中医医院）肾病科
  • 出版日期:2017-12-25 发布日期:2018-11-19
  • 通讯作者: 孙响波，E-mail：sunxiangbo1982@163.com
  • 作者简介:孙响波（1982-），男，医学博士，主治医师。研究方向：中医药治疗肾病的临床与实验研究
  • 基金资助:
    山东省自然科学基金项目（2012ZRB14098）

Effect of Shenzong granule on micro-inflammation in the rats with primary nephrotic syndrome

SUN Xiangbo，YU Nina，PAN Yueli   

  1. Qingdao Haici Medical Group，Qingdao 266000，China
  • Online:2017-12-25 Published:2018-11-19

PDF (PC)

97

摘要：
目的
探讨肾综颗粒对肾病综合征大鼠微炎症的影响。
方法
将40只SPF级雄性Wistar大鼠随机选取8只大鼠作为正常组，其余32只大鼠制备肾病综合征模型，造模成功后随机分为模型组、激素组、肾综颗粒组、激素加肾综颗粒组，每组8只。除正常组、模型组外，其余各组分别予激素、肾综颗粒、激素加肾综颗粒干预，检测血清核因子κB（NF-κB）、白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）、C反应蛋白（CRP）水平。
结果
激素加肾综颗粒组能显著降低肾病综合征大鼠血清NF-κB、IL-1β、TNF-α、IL-6、CRP水平，差异均有统计学意义（P<0.05）。
结论
激素加肾综颗粒组对肾病综合征大鼠微炎症状态具改善作用，其作用机制可能是通过降低IL-1β、TNF-α水平，减少IL-6的分泌，使C反应蛋白、NF-κB含量降低等减慢炎症因子的致炎效应，阻断炎症进程。

关键词: 肾病综合征, 肾综颗粒, 微炎症, 大鼠

Abstract:
Objective
To study the effect of Shenzong granule on micro-inflammation in the rats with nephritic syndrome.
Methods
Of 40 SPF male Wistar rats,8 were chosen randomly as the normal control group (group A),and the remained 32 rats were made into nephrotic syndrome models,which were then divided into 4 groups with 8 in each group:model group(B),hormone group(C),Shenzong group(D) and hormone plus Shenzong group(E).Group C，D and E were intervened respectively with hormone,Shenzong granule and hormone plus shenzong granule;detect the level of serum neuclear factor κB（NF-κB）,interleukin 1β(IL-1β),tumor necrosis factor α（TNF-α）,IL-6,and C-reactive protein(CRP).
Results
The level of NF-κB,IL-1β,TNF-α,IL-6 and CRP in rats with nephrotic syndrome was significantly reduced in group E,and the difference was statistically significant (P<0.05).
Conclusion
The micro-inflammation in rats with nephrotic syndrome is improved by hormone and Shenzong granule. Its working mechanism may be through reducing IL-1β and TNF-α level,the IL-6 secretion,and decreasing the content of CRP and NF-κB,which results in slowing down the reaction of inflammation factors and blocking the inflammation.

Key words: Nephrotic syndrome, Shenzong granule, Micro-inflammation, Rat