Abstract:
Objective
To observe the influence of placenta-derived mesenchymal stem cells on the secretion of IL-4 and IFN-γ in activated T lymphocytes, and to predict the possibility of applying the placenta-derived mesenchymal stem cells in the clinical treatment of various diseases.
Methods
T lymphocytes were isolated from two healthy peoples to make MLC system. The placenta-derived mesenchymal stem cells were obtained, which were co-cultured with MLC system. They were divided into four groups: homologous T cells group Ⅰ, homologous T cell group Ⅱ, co-culture group and positive control group, and each group was inoculated with 10 holes. The culture lasted for 72h. ELISA was used to detect the content of IL-4 and IFN-γ in the cell culture supernatant of each group.
Results
There was no significant difference in IL-4 and IFN-γ in the supernatant between homologous groupⅠand group Ⅱ(P>0.05). compared with group Ⅰ and group Ⅱ, the concentration of IL-4 and IFN-γ was significantly increased in co-culture group and positive control group(P<0.01). Compared with co-culture group, the IL-4 concentration in positive control group was significantly increased, while IFN-γ concentration was significantly decreased, and the difference was statistically significant(P<0.01).
Conclusion
The placenta-derived mesenchymal stem cells can inhibit the ability of activated T lymphocytes to secrete IL-4 and to promote IFN-γ. They may have the ability to promote Th0 to differentiate into Th1.

Key words: Human placenta-derived mesenchymal stem cells, MLC system, Co-culture, IL-4, IFN-γ