ISSN 1674-3865  CN 21-1569/R
主管:国家卫生和计划生育委员会
主办:中国医师协会
   辽宁省基础医学研究所
   辽宁中医药大学附属医院

Chinese Pediatrics of Integrated Traditional and Western Medicine ›› 2019, Vol. 11 ›› Issue (2): 108-111.doi: 10.3969/j.issn.1674-3865.2019.02.005

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Effect of early intervention on the total volume of the myelinated fibers in the white matter of neonatal rat with brain injury due to intrauterine infection

LI Peichun,LUO Nan,LI Xiaojie   

  1. Weifang Traditional Chinese Hospital,Weifang 261041,China
  • Online:2019-04-25 Published:2019-05-08

Abstract:
Objective
To study the effect of early intervention on the total volume of the myelinated fibers in the white matter of neonatal rat with brain injury due to intrauterine infection.
Methods
Prepare the models of brain injury due to intrauterine infection and divide them into two groups:30 Wistar rats at 17d of gestation were given abdominal injection of lipopolysaccharide[450 μg/(kg·d)] for 2 days in a row(the LPS group);6 rats were given the same amount of saline(the saline group). The rats in LPS group were divided randomly into intervention group and no-intervention group. Touching and environment stimulation was given to the intervention group at an early stage, and rehabilitation training at a late stage. The other groups were given conventional feeding. A quantitative research was performed on the total volume of the myelinated fibers in the white matter of the above groups by transmission electron microscope and stereological methods at 14d and 28d of delivery.
Results
(1)The total volume of myelinated fibers in saline group increased from 12.99 mm3 to 32.77 mm3,with an increase rate of 61.5%,from 14d to 28d after delivery(P<0.05). (2)Compared with the saline group, the total volume decreased by 43.5% at 14d after delivery in the no-intervention group, and 34.4% in the intervention group; there was no statistically significance difference in volume between intervention group and nointervention group(P>0.05). (3)On the 28th day after delivery, the total volume of myelinated fibers decreased by 81.3% in the no-intervention group and by 57.8% in the intervention group, compared with the saline group; there was statistical difference in the volume between intervention group and nointervention group(P<0.05).
Conclusion
(1)With age increasing, the total volume of myelinated fibers in normal white matter increases. (2)The brain injury due to intrauterine infection decreases the total volume of the myelinated fibers in white matter, and it becomes more significant with development. (3)Early intervention can increase the total volume of myelinated fibers in white matter.

Key words: Brain injury, White matter, Myelinated fibers, Early intervention, Stereology, Rat