Abstract:
Objective
To establish a method for the simultaneous determination of six components (prim-O-glucosylcimifugin，calycosin-7-glucoside， 5-O-methylvisammioside， baicalin， psoralen and angelicin) in Xiaoer Bushen Gubiao granule by HPLC.
Methods
The chromatographic conditions were determined by high performance liquid chromatography with C18 column (250 mm×4.6 mm，5 μm)，mobile phase being acetonitrile-0.2% formic acid aqueous for solution gradient elution; flow rate was 1.0 mL/min，and column temperature was 30 ℃， injection volume 10 μL; DAD detector was used with the detection wavelength being 254 nm and 280 nm.
Results
The six components were well separated and showed a good linear relationship with peak area (r≥0.999 0) within their respective concentration ranges. The average sample recovery rates of prim-O-glucosylcimifugin，calycosin-7-glucoside， 5-O-methylvisammioside， baicalin， psoralen and angelicin were 99.89%，101.86%，103.22%，99.59%，98.37%，98.12%，and RSD was 3.84%，2.80%，3.12%，4.29%，4.53% and 4.87%， respectively.
Conclusion
The method is accurate， simple， reproducible and sensitive. It can be used for the quality control of multi-components in Xiaoer Bushen Gubiao granule.

Key words: Xiaoer Bushen Gubiao granules, Prim-O-glucosylcimifugin, Calycosin-7-glucoside, 5-O-methylvisammioside, Baicalin, Psoralen, Angelicin, HPLC