ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2015, Vol. 7 ›› Issue (1): 17-19.doi: 10.3969/j.issn.1674-3865.2015.01.007

• 实验论著 • 上一篇    下一篇

大柴胡汤对高脂高胆固醇大鼠TNFα、IL6表达水平的影响

常一川，王凤荣   

  1. 110032 沈阳，辽宁中医药大学研究生学院（常一川）；辽宁中医药大学附属医院心内科（王凤荣）
  • 发布日期:2018-11-19
  • 通讯作者: 王凤荣，110032 沈阳，辽宁中医药大学附属医院心内科。
  • 作者简介:常一川（1987-），男，辽宁中医药大学2012级硕士研究生在读。研究方向：中医药防治心系疾病。

Dachaihu Decoction on high fat and high cholesterol in rats TNFα，IL6 expression level influence.

CHANG Yichuan，WANG Fengrong.   

  1. Graduate School of Liaoning University of Traditional Chinese Medicine，110032 Shenyang，China
  • Published:2018-11-19

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摘要：
目的:通过研究大柴胡汤对高脂高胆固醇大鼠模型心肌组织内肿瘤坏死因子α（TNFα）、白细胞介素6（IL6）蛋白表达的影响，进一步阐明大柴胡汤治疗高脂高胆固醇疾病的作用机制。
方法:将45只幼龄Wistar大鼠随机分为正常对照组、模型组、大柴胡汤组3组。正常对照组正常饮食并每日给予生理盐水灌胃；大柴胡汤组、模型组以高脂饲料喂饲大鼠造模；连续10 d；实验第11天，大柴胡汤组根据给药剂含生药量为0.3 g/mL干预。每日观察大鼠的生存状态，分别在造模后10、20、30 d，测量体质量并统计。实验第30天，采用免疫组化法检测各组大鼠心肌组织内TNFα、IL6蛋白的表达。
结果:与正常对照组比较，高脂高胆固醇模型组心肌组织内TNFα、IL6蛋白表达均显著上调，差异有统计学意义（P＜0.05）。与模型组比较，大柴胡汤组心肌组织内TNFα、IL6蛋白表达明显下降，差异有统计学意义（P＜0.05）。
结论:高脂高胆固醇能刺激机体内TNFα、IL6细胞因子的大量释放，大柴胡汤能较好的抑制TNFα、IL6蛋白表达，对高脂高胆固醇疾病有一定的防治效果。

关键词: 高脂血症/IL6/TNF&alpha, /大柴胡汤/动物/实验/大鼠

Abstract:
Objective:Dachaihutang by studying the impact of high fat and cholesterol in the rat model of myocardial tissue and tumor necrosis factor α（TNFα），interleukin6（IL6）protein expression，and further clarify the treatment of high fat Dachaihutang cholesterol mechanisms of disease.
Methods:45 young Wistar rats were randomly divided into normal control group，model group，Dachaihutang 3 groups. Establish control group，given a normal diet and daily normal saline; hyperlipidemia model groups of rats fed with high fat diet modeling; continuous 10 d; experimental section 11 d，Dachaihutang groups according to the amount of drugcontaining crude drug Health and medicine about 0.3 g/mL intervention. Daily living conditions observed in rats，respectively，after modeling 10，20，30 d，measuring weight and statistics. Experimental Section 30 d，within each group to detect the expression of myocardial tissue TNFα，IL6 protein was detected by immunohistochemistry.
Results:Compared with the normal group，model group within hypercholesterolemic myocardium TNFα，IL6 protein expression were significantly increased（P<0.05）. Compared with the model group，within Dachaihutang myocardial tissue TNFα，IL6 protein expression was significantly decreased（P<0.05）.
Conclusion:The highfat highcholesterol in the body to stimulate TNFα，IL6 massive release of cytokines，Dachaihutang can better inhibit TNFα IL6 protein expression of high fat and cholesterol have a certain disease control effect.

Key words: High cholesterol/IL6/TNF&alpha, /Dachaihu Decoction/animal experiments/rat