ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2019, Vol. 11 ›› Issue (2): 139-143.doi: 10.3969/j.issn.1674-3865.2019.02.013

• 临床研究 • 上一篇    下一篇

小儿紫癜疹消颗粒对过敏性紫癜患儿血清IgA1诱导人脐静脉内皮细胞炎症的抑制机制研究

朱浩宇，段晓征，冯晓纯   

  1. 130021长春，长春中医药大学附属医院儿童诊疗中心
  • 出版日期:2019-04-25 发布日期:2019-05-08
  • 通讯作者: 朱浩宇，E-mail:doctorzhuhaoyu@163.com
  • 作者简介:朱浩宇(1980-)，男，医学博士，副教授。研究方向：中医药治疗儿童自身免疫性疾病
  • 基金资助:
    吉林省科技厅课题项目(20160101175JC)；吉林省教育厅课题项目(吉教科合字［2016］第9号)

Inhibitory effect of Xiao'er Zidian Zhenxiao granule on inflammation of human umbilical vein endothelial cells induced by serum IgA1 in children with HenochSchonlein purpura and its mechanism

ZHU Haoyu，DUAN Xiaozheng，FENG Xiaochun   

  1. Children's Medical Center，Affiliated Hospital of Changchun University of Traditional Chinese Medicine，Changchun 130021，China
  • Online:2019-04-25 Published:2019-05-08

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摘要：
目的探讨小儿紫癜疹消颗粒对过敏性紫癜(HSP)患儿血清IgA1诱导人脐静脉内皮细胞(HUVECs)炎症的抑制作用，并初步阐明其作用机制。
方法以HUVECs为研究对象，用健康儿童血清IgA1和HSP患儿血清IgA1进行干预，同时加入不同浓度的小儿紫癜疹消颗粒，分为空白对照组、正常对照组、HSP组及低、中、高浓度中药组(25、50、100 mg/L)，各组于培养24 h后，采用酶联免疫吸附法测定各组上清液中肿瘤坏死因子α(TNF-α)、白细胞介素-8(IL-8)水平。Westernbolt检测各组细胞内核因子κB(NF-κB)蛋白表达水平。
结果各组细胞上清液中TNF-α、IL-8水平，与空白对照组和正常对照组相比，HSP组显著升高，差异有统计学意义(P<0.001)；与HSP组比较，低、中、高浓度中药组TNF-α、IL-8水平明显降低，差异有统计学意义(P<0.05)。Western blot检测各组HUVECs P65蛋白表达水平，与空白对照组和正常对照组比较，HSP组P65蛋白显著升高，差异有统计学意义(P<0.05)；与HSP组比较，高浓度中药组P65蛋白水平明显降低，差异有统计学意义(P<0.05)。
结论小儿紫癜疹消颗粒可使HUVECs中NFκB信号通路P65蛋白的磷酸化水平降低，减少相关炎症因子TNF-α、IL-8的分泌，从而有效抑制细胞炎症反应的发生。

关键词: 过敏性紫癜, 小儿紫癜疹消颗粒, 人脐静脉内皮细胞, 核因子κB

Abstract:
Objective
To investigate the inhibitory effect of Xiao'er Zidian Zhenxiao granules on the inflammation of human umbilical venous endothelial cells(HUVECs) induced by serum IgA1 in children with HenochSchnlein purpura(HSP), and to clarify its mechanism.
Methods
HUVECs were used as the research subjects, serum IgA1 in HSP children and in healthy children were used to intervene, and different concentrations of Xiao'er Zidian Zhenxiao granules were added. They were divided into blank control group, normal control group, Henoch-Schonlein purpura group(HSP group) and the Xiao'er Zidian Zhenxiao granules groups(ZXKL groups)(the low-,medium-and high-concentration group, with the concentration being 25 mg/L, 50 mg/L and 100 mg/L respectively). After 24 hours of culturing in each group, TNF-α and IL-8 levels in the supernatant of each group were determined by enzyme-linked immunosorbent assay. Westernbolt was used to detect the expression of NF-κB protein in each group.
Results
The levels of TNF-α and IL-8 in the supernatant of each group were significantly higher in the HSP group than in the blank and normal control groups(P<0.001). Compared with the HSP group, the levels of TNF-α and IL-8 in the ZXKL groups decreased(P<0.05). The expression of P65 protein in HUVECs was detected by Western blot. Compared with the blank and normal control groups, the P65 protein level in the HSP group was significantly increased(P<0.05). Compared with the HSP group, the P65 protein level in the high-concentration ZXKL group(100 mg/L) was decreased(P<0.05).
Conclusion
Xiao'er Zidian Zhenxiao granules can decrease the phosphorylation of NF-κB signaling pathway P65 protein in HUVECs and reduce the secretion of related inflammatory cytokines(TNF-α and IL-8), thus effectively inhibiting the occurrence of cellular inflammatory responses.

Key words: Henoch-Schonlein purpura, Xiao'er Zidian Zhenxiao granules, Human umbilical venous endothelial cell, Nuclear factor-κB