ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2020, Vol. 12 ›› Issue (2): 109-112.doi: 10.3969/j.issn.1674-3865.2020.02.005

• 实验论著 • 上一篇    下一篇

虎杖提取物对肺炎支原体感染后小鼠心肌损伤的干预作用

吕佳佳，姜永红   

  1. 200032上海，上海中医药大学附属龙华医院儿科
  • 出版日期:2020-04-25 发布日期:2021-05-17
  • 通讯作者: 姜永红，E-mail:jyh203225@126.com
  • 作者简介:吕佳佳(1991-),女,医学硕士，医师。研究方向：中医药防治小儿呼吸系统疾病
  • 基金资助:
    上海市科学技术委员会科研计划项目（19401933600）

Intervention effect of Polygonum cuspidatum extract on myocardial injury in mice with Mycoplasma pneumoniae infection

  • Online:2020-04-25 Published:2021-05-17

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摘要： 目的 研究虎杖提取物对肺炎支原体（MP）感染后小鼠心肌损伤的干预作用。
方法 30只BALB/c小鼠随机分为正常组、MP模型组、虎杖提取物组，每组10只。正常组用生理盐水滴鼻，MP模型组和虎杖提取物组均用支原体菌液滴鼻感染，连续3 d。治疗从感染第1天开始，连续治疗3 d。ELISA法检测小鼠血清中肌酸激酶同工酶（CK-MB）、心肌肌钙蛋白（cTnI）、肌红蛋白（Mb）和谷草转氨酶（AST）的表达水平。结果 与正常组相比，MP模型组的心肌损伤标志物水平均显著升高，差异有统计学意义（P<0.01）；与MP模型组相比，虎杖提取物组显著下调了CK-MB、cTnI、Mb、AST的水平，差异有统计学意义（P<0.01）。
结论 虎杖提取物能降低心肌损伤标志物水平，显著改善MP感染后小鼠的心肌损伤，起到保护心脏的作用。

关键词: 支原体肺炎, 心肌损伤, 虎杖提取物, 小鼠

Abstract: Objective  To study the effect of Polygonum cuspidatum extract on myocardial injury in mice with Mycoplasma pneumoniae infection.
Methods Totally 30 BALB/c mice were randomly divided into normal group, MP model group and Polygonum cuspidatum extract group, with 10 mice in each group. The normal group was nasally infused with normal saline, and the MP model group and Polygonum cuspidatum extract group were all nasally infected with mycoplasma bacteria solution for 3 consecutive days. Treatment started on the first day of infection and continued for 3 days. The expression level of creatine kinase isoenzyme(CK-MB), cardiac troponin(cTnI), myoglobin(Mb) and aspartate aminotransferase(AST) in serum of mice were detected by ELISA method.
Results Compared with the normal group, the levels of all the myocardial injury markers in the MP model group were significantly increased(P<0.01); compared with the MP model group, in the Polygonum cuspidatum extract group CK-MB, cTnI, Mb and AST levels were significantly down-regulated(P<0.01).
Conclusion Polygonum cuspidatum extract can decrease the levels of CK-MB, cTnI, Mb and AST, significantly improve myocardial damage in mice with Mycoplasma pneumoniae infection, and protect the heart.

Key words: Mycoplasma pneumoniae pneumonia, Myocardial injury, Polygonum cuspidatum extract, Mice