ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2018, Vol. 10 ›› Issue (2): 109-113.doi: 10.3969/j.issn.1674-3865.2018.02.006

• 临床研究 • 上一篇    下一篇

外周血淋巴细胞亚群检测对儿童亚急性坏死性淋巴结炎的诊疗意义

朱林超，张书峰，王旭辉，王霖，王晓晖，孙英   

  1. 450003 郑州，郑州大学人民医院，河南省人民医院小儿外科（朱林超，张书峰，王旭辉，王霖，王晓晖）；450006 郑州，河南省直第三人民医院检验科（孙英）
  • 出版日期:2018-04-25 发布日期:2018-11-19
  • 通讯作者: 王旭辉，E-mail：zlcmusic@163.com
  • 作者简介:朱林超（1983-），男，医学硕士，主治医师。研究方向：小儿外科疾病的基础与临床研究
  • 基金资助:
    河南省医学科技攻关计划普通项目（201702168）

Significance of peripheral blood lymphocyte subsets detection in the diagnosis and treatment of children with subacute necrotizing lymphadenitis

ZHU Linchao，ZHANG Shufeng，WANG Xuhui，WANG Lin，WANG Xiaohui，SUN Ying   

  1.  Department of Pediatric Surgery，Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003,China
  • Online:2018-04-25 Published:2018-11-19

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摘要：
目的
探讨外周血淋巴细胞亚群检测对儿童亚急性坏死性淋巴结炎（CSNL）的诊疗意义。
方法
选取2013年1月至2016年12月河南省人民医院小儿外科及内科收治的CSNL患儿51例为CSNL组，同期选择门诊健康体检儿童30例为对照组。采用流式细胞技术检测两组受试对象外周血T淋巴细胞亚群百分率，并观察CSNL治疗效果。
结果
51例CSNL患儿中，治愈38例，好转12例，无效1例。治疗前CSNL患儿CD3+、CD4+、CD19+淋巴细胞亚群百分率和CD4+/CD8+均显著低于对照组，差异有统计学意义(P<0.05)。38例治愈患儿和12例好转患儿治疗中、后外周血CD3+、CD4+、CD19+淋巴细胞亚群百分率和CD4+/CD8+显著高于治疗前，治疗后的指标又显著高于治疗中，差异有统计学意义(P<0.05)。
结论
细胞亚群CD3+、CD4+、CD4+/CD8+及CD19+，可作为临床CSNL诊疗的重要评估指标。

关键词: 亚急性坏死性淋巴结炎, 淋巴细胞亚群, CD3+, CD4+, CD4+, CD8+, CD19+, 儿童

Abstract:
objective
To investigate the significance of peripheral blood lymphocyte subsets detection in the diagnosis and treatment of children with subacute necrotizing lymphadenitis(CSNL).
Methods
The percentage of lymphocyte subsets in peripheral blood of 51 children with CSNL(CSNL group) and 30 healthy children(control group) was detected by flow cytometry(FCM), and the treatment results of CSNL were observed.
Results
In the 51 cases of CSNL,38 were cured,12 improved and 1 failed. Before treatment, the percentage of CD3+,CD4+ and CD19+ of lymphocyte subsets and CD4+/CD8+ of CSNL group were significantly lower than control group(P<0.05).The percentage of CD3+,CD4+ and CD19+ of lymphocyte subsets and CD4+/CD8+ of the 51 children with CSNL were significantly higher during and after treatment than before treatment(P<0.05),and the index after the treatment was higher than that during treatment(P<0.05).
Conclusion
The CD3+,CD4+ and CD19+ of peripheral blood lymphocyte subsets and CD4+/CD8+ can be used as important indicators for clinical diagnosis and treatment of CSNL.

Key words: CSNL, Lymphocyte subsets, CD3+\CD4+, CD4+, CD8+, CD19+, Child