ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2010, Vol. 2 ›› Issue (1): 8-12.doi: 10.3969/j.issn.1674-3865.2010.01.004

• 专栏 • 上一篇    下一篇

早期行为疗法降低仔鼠脑损伤致脑性瘫痪的实验研究

庞伟，李晓捷，张士岭
  

  1. 154003 黑龙江 佳木斯，佳木斯大学附属第三医院小儿神经科(庞伟),小儿脑瘫科(李晓捷);佳木斯大学儿童神经康复实验室(庞伟,李晓捷,张士岭)
  • 出版日期:2010-02-25 发布日期:2019-06-03
  • 通讯作者: 李晓捷，154003 黑龙江 佳木斯，佳木斯大学附属第三医院小儿脑瘫科。
  • 作者简介:庞伟（1976-），男，主治医师。研究方向：小儿脑损伤发病机制及早期防治研究。

Experimental study of the early behavior therapy to reduce cerebral palsy of the young rats due to brain damage

PANG Wei，LI Xiaojie，ZHANG Shiling.   

  1. Department of Pediatric Neurology，the Third Affliated Hospital of Jiamusi University，Jiamusi 154003,China
  • Online:2010-02-25 Published:2019-06-03

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摘要： 目的研究早期行为疗法对降低仔鼠脑损伤所致脑性瘫痪（简称脑瘫）的影响。
方法取孕18 d大鼠21只连续2 d腹腔注射脂多糖，剂量为450 μg/kg，6只孕鼠腹腔注射同剂量的生理盐水。随机选取脂多糖组仔鼠分为干预组（n=20）和非干预组（n=30），另设生理盐水组（n=30）；生后2 d起，给予干预组仔鼠早期丰富环境刺激等行为疗法，另两组常规饲养；于生后25 d对3组仔鼠进行随意运动、姿势、肌张力、不自主运动、情感行为能力、倾斜板试验等检测，记录各项评分并进行统计学分析；取生后1 d的非干预组、生理盐水组仔鼠各10只、生后25 d的3组仔鼠各10只进行脑组织髓鞘碱性蛋白(myelin basic protein，MBP)、S100蛋白阳性细胞检测。
结果免疫组织化学显示：生后1 d的非干预组仔鼠脑组织各部位(内囊、海马、胼胝体)的MBP阳性染色较生理盐水组仔鼠均明显减少、减弱(P<0.01)；生后25 d的干预组仔鼠脑组织各部位(内囊、海马、胼胝体)的MBP阳性染色较非干预组仔鼠均增多(P<0.05),生理盐水组仔鼠脑组织各部位(内囊、海马、胼胝体)的MBP阳性染色较干预组、非干预组仔鼠明显增多(P<0.01)；生后1，25 d的非干预组仔鼠脑组织各部位(内囊、海马、胼胝体)的S100蛋白阳性染色与生理盐水组仔鼠相比较，均明显增加、增强（P<0.01），干预组介于两者之间，差异有统计学意义（P<0.01）。神经行为学检测显示：干预组20只仔鼠中有4只鉴定为脑瘫鼠，非干预组有8只脑瘫鼠，而生理盐水组无脑瘫鼠。
结论MBP、S100蛋白可以作为脑损伤早期诊断及判断预后的指标；早期干预可降低宫内感染所致新生鼠脑瘫的发生。

关键词: 脑性瘫痪, 脑损伤, 行为疗法, 大鼠, 动物, 实验

Abstract: ObjectiveTo study the effect of early behavior therapy to reduce cerebral palsy caused by brain damage in rats.
MethodsTotally 27 pregnant rats were consecutively injected with LPS(450 μg/kg，n=21) or saline(n=6) on gestation 18 d and 19 d.We selected randomly saline neonatal rat group B(n=30) and LPS neonatal rat group A1(n=20)，A2(n=30).The 2 d rats(A1) were given early behavior therapy，such as enriched environment and the rats(A2 and B) were routinely fed.The neurobehavior of the rats was observed in A1，A2 and B groups on 25 d.The MBP and S100 were detected in brain sections on 1 d and 25 d.
ResultsMBP expression was obviously decreased in A2(1 d),compared with B of 1 d rats(P<0.01);MBP was increased in A1 of 25 d,compared with A2 of 25 d(P<0.05)；it was increased in B,contrasted with A1 and A2(P<0.01).Compared with B,there was significant increase in A2 of 1 d with regard to S100(P<0.01).The result of S100 between A2 and B of 25 d was the same with 1 d rats,but the S100 of A1 was the middle of them（P<0.01）．The result of neurobehavioral observation indicated that there were 4 cerebral palsy rats in A1,8 cerebral palsy rats in A2 and no cerebral palsy rat in B.
ConclusionMBP and S100 can become the sign of early diagnosis and the therapeutic effect in brain injury.Early interference can reduce cerebral palsy.

Key words: Cerebral palsy;Brain damage;Behavior therapy;Rat；Animal, experiment