ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2010, Vol. 2 ›› Issue (2): 132-134.doi: 10.3969/j.issn.1674-3865.2010.02.015

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太子健Ⅱ煎剂对哮喘模型豚鼠嗜酸性粒细胞及内皮素1的影响

李岚，陈华，俞景茂   

  1. 310006，杭州，浙江中医药大学附属第一临床医学院儿科
  • 收稿日期:2010-01-10 出版日期:2010-04-25 上线日期:2010-04-25
  • 通讯作者: 俞景茂
  • 作者简介:李岚（1971-），女，医学硕士，讲师、主治医师。研究方向：中西医结合治疗小儿呼吸系统疾病。

Effects of TaizijianⅡ decoction on EOS and ET-1 in guinea pigs of allergic asthma

LI Lan，CHEN Hua,YU Jing mao.   

  1. Department of Pediatrics，The First Clinical College of Zhejiang Chinese Medical University，Hangzhou 310006,China
  • Received:2010-01-10 Published:2010-04-25 Online:2010-04-25

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摘要： 目的：观察太子健Ⅱ煎剂对哮喘模型豚鼠支气管壁内的嗜酸性粒细胞（EOS）及内皮素1（ET-1）的影响，探讨太子健Ⅱ煎剂抗小儿哮喘复发的作用机制。方法：将50只豚鼠随机分为5组，正常对照组、哮喘模型组、地塞米松组、高、低剂量太子健Ⅱ煎剂组，卵白蛋白致敏并成功诱喘，分别灌服生理盐水、地塞米松、高、低剂量太子健Ⅱ煎剂，连续15 d。用计数板计数豚鼠血、支气管肺泡灌洗液（BALF）、支气管壁内的EOS数，放免法检测BALF中ET-1含量。结果：与哮喘模型组比较，太子健Ⅱ煎剂高剂量组能减少BALF及支气管壁内EOS的数量（P＜0.05），降低BALF中ET-1的水平（P＜0.01）。结论：太子健Ⅱ煎剂可能通过减轻EOS浸润、ET-1产生，减轻气道的慢性炎症。

关键词: 哮喘；太子健Ⅱ煎剂；豚鼠；动物, 实验；嗜酸细胞；内皮素缩血管肽类

Abstract: Objective：To observe the effect of TaizijianⅡ decoction（TZJⅡ） on EOS and ET-1 of guinea pig asthma models.〖JP2〗To explore the mechanism of TZJⅡ on anti-relapsing children asthma.〖WT5"HZ〗Methods：50-guinea pigs were divided into 5 groups randomly，normal control group，asthma model group，dexamethasone group，high-dose TZJⅡ group and low-dose TZJⅡ group.The five groups were sensitized with ovalbumin，and induced asthma successfully.After that each group were given different preventive and treatments for 15 days.Then the number of eosinophils（EOS） in peripheral blood，bronchoalveolar lavage fluid（BALF） and bronchial wall were counted by counting plate.The content of ET-1 in BALF were measured by radioimmunity methods.〖WT5"HZ〗Results：High-dose TZJⅡ could reduce the number of EOS in 〖JP2〗BALF and bronchial wall（P<0.05），lower the ET- content in BALF（P<0.01） compared with guinea pig asthma models.〖WT5"HZ〗Conclusion：TZJⅡ- can lessen EOS infiltration and regulate ET-1 production，suggesting that it might take effect in alleviating the chronic airway inflammation.

Key words: Asthma；TaizijianⅡdecoction；Guinea pig；Animal, experiment, Eosinophils；Endothelin