ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2013, Vol. 5 ›› Issue (1): 21-23.doi: 10.3969/j.issn.1674-3865.2013.01.006

• 实验研究专栏 • 上一篇    下一篇

胰岛素样生长因子1对单侧输尿管梗阻大鼠肾间质纤维化的影响

张宏，王丙增，张旭亚，孟凡威   

  1. 475001 河南 开封，河南大学第一附属医院儿科
  • 出版日期:2013-02-25 发布日期:2019-04-25
  • 作者简介:张宏（1978-），男，医学硕士，主治医师。研究方向：器官纤维化，Email：zhanghong20048088@126.com。

Effect of IGF1 on renal interstitial fibrosis in rats with unilateral ureteral obstruction

ZHANG Hong，WANG Bingzeng，ZHANG Xuya，et al.   

  1.  Department of pediatrics, The First Affiliated Hospital of Henan University，Kaifeng 475001，China
  • Online:2013-02-25 Published:2019-04-25

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摘要：
目的:观察胰岛素样生长因子1（IGF1）对单侧输尿管梗阻大鼠肾间质纤维化的影响。
方法:30只Wistar大鼠随机分为假手术组、模型组、IGF1组，每组10只。应用单侧输尿管结扎制备肾间质纤维化大鼠模型。假手术组打开腹腔后即缝合；模型组每日腹腔注射生理盐水0.5 mL；IGF1组腹腔注射重组人IGF1 750 ng/(kg·d)。术后第14天麻醉大鼠后取材，检测肾功能，观察Ⅲ型胶原蛋白、TGFβ1、PAI1的表达。
结果:模型组血肌酐、尿素氮、Ⅲ型胶原蛋白、TGFβ1、PAI1的表达水平均高于假手术组，差异有统计学意义（P＜0.05）；IGF1干预后，IGF1组血尿素氮、血肌酐、Ⅲ型胶原蛋白、TGFβ1、PAI1的表达水平均高于假手术组及模型组，差异有统计学意义（P＜0.05）。
结论:IGF1参与梗阻性肾病的病理生理过程，尤其在肾间质纤维增生过程中起着重要作用。

Abstract:
Objective：To investigate the effect of IGF1 on renal interstitial fibrosis in rats with unilatreal ureteral obstruction(UUO).
Methods：Totally 30 rats were randomly divided into 3 groups, with 10 rats in each group, namely, sham group, model group and IGF1 group. Renal interstitial fibrosis models were made by unilateral ureteral ligation. In the sham group, the abdomen was opened and then stitched; in the model group, physiological saline was intragastrically injected, 0.5 mL a day; in IGF1 group, recombinant human IGF1 was intragastrically injected, 750 mg/(kg·d). At the 14th day after the operation, the rats were anesthetized, and related materials were taken, the kidney function was tested, and   ColⅢ，TGFβ1 and PAI1 expressions in kidneys were observed.
Results：The expression of BUN，Scr，ColⅢ，TGFβ and PAI1 in the model group were significantly higher than those in sham group, the difference having statistic significance. After the intervention of IGF1, the level of BUN，Scr，ColⅢ，TGFβ and PAI1 in IGF1 group was significantly higher than that in sham and model group, the difference had statistic significance（P＜0.05）.
Conclusions：IGF1 plays a role in the pathophysiological process of obstructive nephropathy，especially in the renal interstitial fibrosis.