ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2016, Vol. 8 ›› Issue (2): 161-163.doi: 10.3969/j.issn.1674-3865.2016.02.011

• 临床研究 • 上一篇    下一篇

Daxx蛋白在儿童急性白血病骨髓细胞中的表达意义

冯静乔   

  1. 515000 广东 汕头，汕头大学医学院第一附属医院儿科
  • 出版日期:2016-04-25 发布日期:2018-11-19
  • 作者简介:冯静乔(1979-)，女，医学硕士，主治医师。研究方向：小儿血液病的基础与临床研究，E-mail：fengjingqiao2015@163.com

Expression significance of Daxx protein in bone marrow cells of children with acute leukemia

FENG Jingqiao   

  1. The First Affiliated Hospital of Shantou University，Shantou 515000，China.
  • Online:2016-04-25 Published:2018-11-19

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摘要：
目的：探究Daxx蛋白在儿童急性白血病骨髓细胞中的表达意义。
方法：2010年2月至2014年10月汕头大学医学院第一附属医院儿科收治住院的急性白血病患儿100例为观察组，又根据疾病类型分为3个亚组，即急性淋巴细胞白血病(ALL)组49例，急性非淋巴细胞白血病(ANLL)组21例，急性髓细胞白血病(AML)组30例。对照组30例，为同年龄正常健康儿童。采用免疫组织化学方法检测Daxx蛋白的表达。
结果：对照组儿童骨髓细胞中Daxx蛋白的表达阳性率与ALL组比较差异无统计学意义(P>0008 3)；对照组儿童骨髓细胞中Daxx蛋白的表达阳性率显著低于ANLL组和AML组，差异有统计学意义(P<0008 3)。对照组中Daxx蛋白在儿童急性白血病骨髓中的表达(601±113)μg/L，显著高于ALL组(355±232)μg/L、ANLL组(354±247)μg/L、AML组(312±156)μg/L，差异有统计学意义(P<005)。
结论：Daxx蛋白在儿童急性白血病骨髓细胞中过度表达，可能对促进白血病细胞的增殖、生长过程起到重要作用。

关键词: 急性白血病, Daxx蛋白, 儿童

Abstract:
Objective:To explore the expression significance of Daxx protein in bone marrow cells of children with acute leukemia.
Methods:Totally 100 cases of children with acute leukemia were selected as the research object in this study from Feb.2010 to Oct.2014 in the First Affiliated Hospital of Shantou University Medical College and divided into 3 groups according to the type of disease,including acute lymphoblastic leukemia(ALL)group of 49 cases,acute non-lymphocytic leukemia(ANLL)group of 21 cases,acute myeloid leukemia(AML)group of 30 cases.The control group consisted of 30 cases,who were normal healthy children of the same age.The expression of Daxx protein was detected by immunohistochemical method.
Results:The expression rate of Daxx protein in the control group(667%)was much smaller than that in ALL group(3038%),ANLL group(6667%)and AML group(4667%),the difference being statistically significant(P<005).Expression of Daxx protein in the control group in the bone marrow of children with acute leukemia(601±113)μg/L was significantly higher than that in ALL group(355±232)μg/L,ANLL group(354±247)μg/L and AML group(351±253)μg/L,the difference being statistically significant(P<005).
Conclusion:The overexpression of Daxx protein in bone marrow cells of children with acute leukemia may play an important role in promoting the proliferation and growth of leukemia cells.

Key words: Acute leukemia, Daxx protein, Children