ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2018, Vol. 10 ›› Issue (1): 36-39.doi: 10.3969/j.issn.1674-3865.2018.01.010

• 临床研究 • 上一篇    下一篇

TBX1基因可能通过调控Tgfb2基因的表达参与心脏发育及先天性心脏病的发生

程菲,姜红堃   

  1. 110001 沈阳，中国医科大学附属第一医院儿科
  • 出版日期:2018-02-25 发布日期:2018-03-23
  • 通讯作者: 姜红堃，E-mail：jianghongkun007@163.com
  • 作者简介:程菲（1981-），女，医学硕士，主治医师，目前工作于沈阳市儿童医院。研究方向：小儿心脏发育
  • 基金资助:
    国家自然科学基金项目（81300130）

TBX1 gene may be involved in heart development and development of congenital heart disease through regulating Tgfb2 gene expression

CHENG Fei，JIANG Hongkun   

  1. Department of Pediatrics，the First Affiliated Hospital of China Medical University，Shenyang 110001，China
  • Online:2018-02-25 Published:2018-03-23

PDF (PC)

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摘要：
目的
通过检测TBX1基因干扰后转化生长因子β2基因（Tgfb2）表达的变化探讨TBX1基因参与心脏发育及先天性心脏病发生的可能途径。
方法
根据处理因素不同，将细胞分为NC-siRNA组和TBX1-siRNA组。在大鼠胚胎心肌细胞H9C2中转染TBX1-siRNA，采用实时定量PCR法（qRT-PCR）检测干扰效率；收集转染TBX1-siRNA 48 h后的H9C2细胞系，提取总RNA，qRT-PCR法检测干扰后Tgfb2基因mRNA的表达水平。
结果
(1)qRT-PCR检测心肌细胞TBX1 mRNA的表达:H9C2细胞转染TBX1-siRNA 48 h后为0.22±0.02，与NC-siRNA(0.99±0.02)比较，TBX1 mRNA表达显著下调，差异有统计学意义（P<0.05）。(2)qRT-PCR检测心肌细胞Tgfb2 mRNA的表达:H9C2细胞转染TBX1-siRNA 48 h后为2.19±0.01，与NC-siRNA(1.00±0.01)比较，Tgfb2 mRNA表达明显上调，差异有统计学意义（P<0.05）。
结论
TBX1基因表达下调可能通过调控Tgfb2基因的表达参与心脏发育及先天性心脏病的发生。

关键词: 先天性心脏病, TBX1基因, Tgfb2基因, 心脏发育, 儿童

Abstract:
Objective
By detecting the expression of transforming growth factor beta2 gene (Tgfb2) after TBX1 gene interference, we explored the possible way of TBX1 gene involving in cardiac development and development of congenital heart disease.
Methods
According to the different treatment factors, the cells were divided into NC-siRNA group and TBX1-siRNA group. TBX1-siRNA was transfected in rat embryonic cardiomyocyte H9C2, and the interference efficiency was detected by real-time quantitative PCR (qRT-PCR). The H9C2 cell line at 48h after transfection with TBX1-siRNA for was collected to extract the total RNA, and qRT-PCR method was used to detect the expression level of Tgfb2 gene mRNA after interference.
Results
(1)qRT-PCR was used to detect the expression of TBX1 mRNA in myocardial cells. The expression was (0.22±0.02) at 48h after transfection with TBX1-siRNA. Compared with NC-siRNA (0.99±0.02), the expression of TBX1 mRNA was significantly down-regulated, and the difference was statistically significant(P<0.05). (2)The expression of Tgfb2 mRNA in myocardial cells was detected by qRT-PCR, and the expression of H9C2 cells was transfected with TBX1-siRNA. After 48h, the expression of Tgfb2 mRNA was significantly up-regulated (2.19±0.01) compared with that of NC-siRNA(1.00±0.01). The difference was statistically significant(P<0.05).
Conclusion
The down-regulation of TBX1 gene expression may be involved in the development of heart and the development of congenital heart disease by regulating the expression of Tgfb2 gene.

Key words: Congenital heart disease, TBX1 gene, Tgfb2 gene, Heart development, Child