早期干预对宫内感染致脑损伤仔鼠大脑白质有髓神经纤维总体积的影响

doi:10.3969/j.issn.1674-3865.2019.02.005

中国中西医结合儿科学 ›› 2019, Vol. 11 ›› Issue (2): 108-111.doi: 10.3969/j.issn.1674-3865.2019.02.005

早期干预对宫内感染致脑损伤仔鼠大脑白质有髓神经纤维总体积的影响

李佩春，罗南，李晓捷

261041 山东潍坊，潍坊市中医院脑病康复科(李佩春)，肿瘤三科(罗南)；154003 黑龙江佳木斯，佳木斯大学附属第三医院脑瘫科(李晓捷)

出版日期:2019-04-25 发布日期:2019-05-08
通讯作者: 李晓捷，E-mail：xiaojljms@vip.163.com
作者简介:李佩春(1982-)，男，医学硕士，副主任医师。研究方向：小儿先天性脑损伤、脑卒中康复
基金资助:
黑龙江省自然科学基金项目(D2004-50)

Effect of early intervention on the total volume of the myelinated fibers in the white matter of neonatal rat with brain injury due to intrauterine infection

LI Peichun，LUO Nan，LI Xiaojie

Weifang Traditional Chinese Hospital，Weifang 261041，China

Online:2019-04-25 Published:2019-05-08

摘要/Abstract

摘要：
目的
探讨早期干预对宫内感染致脑损伤仔鼠脑白质有髓神经纤维总体积的影响。
方法
制备宫内感染致脑损伤模型及分组：给予30只孕17 d Wistar大鼠连续2 d腹腔注射脂多糖450 μg/(kg·d)，为脂多糖组；6只注射同等剂量的生理盐水设为对照组。将脂多糖组所产脑损伤仔鼠又随机分为干预组及非干预组。干预组早期进行触摸及环境刺激，晚期进行康复训练。其余各组常规饲养。分别于生后14、28 d运用投射电子显微镜和体视学方法对上述各组的脑白质有髓神经纤维总体积进行定量研究。
结果
(1)生理盐水组从生后第14～28天，有髓神经纤维总体积从12.99 mm3增加到32.77 mm3，增加了61.5%(P<0.05)。(2)同生理盐水组比较，在第14天非干预组有髓神经纤维总体积减少了43.5%，干预组减少了34.4%；非干预组与干预组有髓神经纤维总体积比较差异无统计学意义(P>0.05)。(3)第28天时，非干预组有髓神经纤维总体积较生理盐水组相比减少了81.3%，干预组减少了57.8%；干预组与非干预组有髓神经纤维总体积比较差异有统计学意义(P<0.05)。
结论
(1)随着年龄的增长，正常脑白质有髓神经纤维的总体积增加；(2)宫内感染致脑损伤使脑白质有髓神经纤维总体积下降，而且随着发育愈明显；(3)早期干预能增加脑白质有髓神经纤维的总体积。

关键词: 脑损伤, 白质, 有髓神经纤维, 早期干预, 体视学, 大鼠

Abstract:
Objective
To study the effect of early intervention on the total volume of the myelinated fibers in the white matter of neonatal rat with brain injury due to intrauterine infection.
Methods
Prepare the models of brain injury due to intrauterine infection and divide them into two groups:30 Wistar rats at 17d of gestation were given abdominal injection of lipopolysaccharide［450 μg/（kg·d）］ for 2 days in a row(the LPS group);6 rats were given the same amount of saline(the saline group). The rats in LPS group were divided randomly into intervention group and no-intervention group. Touching and environment stimulation was given to the intervention group at an early stage, and rehabilitation training at a late stage. The other groups were given conventional feeding. A quantitative research was performed on the total volume of the myelinated fibers in the white matter of the above groups by transmission electron microscope and stereological methods at 14d and 28d of delivery.
Results
(1)The total volume of myelinated fibers in saline group increased from 12.99 mm3 to 32.77 mm3,with an increase rate of 61.5％，from 14d to 28d after delivery(P<0.05). (2)Compared with the saline group, the total volume decreased by 43.5% at 14d after delivery in the no-intervention group, and 34.4% in the intervention group; there was no statistically significance difference in volume between intervention group and nointervention group(P＞0.05). (3)On the 28th day after delivery, the total volume of myelinated fibers decreased by 81.3％ in the no-intervention group and by 57.8％ in the intervention group, compared with the saline group; there was statistical difference in the volume between intervention group and nointervention group(P<0.05).
Conclusion
(1)With age increasing, the total volume of myelinated fibers in normal white matter increases. (2)The brain injury due to intrauterine infection decreases the total volume of the myelinated fibers in white matter, and it becomes more significant with development. (3)Early intervention can increase the total volume of myelinated fibers in white matter.

Key words: Brain injury, White matter, Myelinated fibers, Early intervention, Stereology, Rat

李佩春，罗南，李晓捷. 早期干预对宫内感染致脑损伤仔鼠大脑白质有髓神经纤维总体积的影响[J]. 中国中西医结合儿科学, 2019, 11(2): 108-111.

LI Peichun，LUO Nan，LI Xiaojie. Effect of early intervention on the total volume of the myelinated fibers in the white matter of neonatal rat with brain injury due to intrauterine infection[J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2019, 11(2): 108-111.

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早期干预对宫内感染致脑损伤仔鼠大脑白质有髓神经纤维总体积的影响

Effect of early intervention on the total volume of the myelinated fibers in the white matter of neonatal rat with brain injury due to intrauterine infection

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