ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2021, Vol. 13 ›› Issue (1): 1-.doi: 10.3969/j.issn.1674-3865.2021.01.001

• 实验论著 •    下一篇

miRNAs小鼠哮喘模型中的作用初探

张冬姣，徐浩   

  1. 110024 沈阳，沈阳医学院附属中心医院儿科
  • 收稿日期:2020-09-08 出版日期:2021-02-25 上线日期:2021-05-17
  • 通讯作者: 徐浩，E-mail:xuhao6668@126.com

Role of miRNAs in the model of asthma in mice

ZHANG Dongjiao，XU Hao   

  1. Pediatric Department，Central Hospital Affiliated to Shenyang Medical Colloge，Shenyang 110024，China
  • Received:2020-09-08 Published:2021-02-25 Online:2021-05-17
  • Contact: XU Hao，E-mail:xuhao6668@126.com

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摘要： 目的：探讨小鼠哮喘模型中miRNAs的差异表达情况。方法：将24只BALB/c小鼠随机分为正常对照组、卵清蛋白处理组、地塞米松治疗组各8只，使用血细胞分析仪检测每组肺泡灌洗液中嗜酸性粒细胞(EOS)、嗜中性粒细胞(NEU)、巨噬细胞(MAC)和淋巴细胞(LYM)的表达情况，通过ELISA的方法检测每组肺泡灌洗液中白细胞介素-4（IL-4）、IL-6和IL-13的表达情况。miRNAs芯片分析不同组别肺组织中差异性表达miRNAs的情况，统计分析miR-455与EOS、NEU、MAC、LYM、IL-4、IL-6和IL-13的相关性。结果：卵清蛋白处理组中EOS、NEU、MAC、LYM、IL-4、IL-6和IL-13的表达显著高于正常对照组，地塞米松治疗组与卵清蛋白处理组相比各种因子表达下调。卵清蛋白处理组多个miRNAs表达发生变化，miR-455在卵清蛋白处理组上调显著。miR455与EOS、NEU、MAC、LYM、IL-4、IL-6和IL-13的表达呈正相关。结论：miR-455在卵清蛋白诱导的小鼠哮喘模型中显著上调。

关键词: 哮喘, 微小RNA, 炎症因子, 细胞因子

Abstract: Objective：To investigate the differential expression of miRNAs in asthma model of mice.Methods：BALB/c mice were randomly divided into normal control group， asthma group(ovalbumin treatment group) and treatment group(dexamethasone treatment group)， 8 mice in each group. Eosinophil(EOS)， neutrophil(NEU)， macrophage(MAC) and lymphocyte(LYM) in BALF of each group were detected by hematology analyzer, and the expression of IL-4，IL-6 and IL-13 in BALF of each group were detected by ELISA. miRNAs microarray was used to analyze the differential expression of miRNAs in different groups of lung tissues; the correlation between miR-455 and EOS，NEU，MAC，LYM，IL-4，IL-6 and IL-13 was statistically analyzed.Results：The expression of EOS，NEU，MAC，LYM，IL-4，IL-6 and IL-13  in the asthma group was significantly higher than that in the control group, and the expression of various factors was downregulated in treatment group compared with asthma group. The expression of multiple miRNAs in asthma group changed，and miR-455 was up-regulated significantly in asthma group. miR-455 was positively correlated with the expression of EOS，NEU，MAC，LYM，IL-4，IL-6 and IL-13.Conclusion：miR-455 is significantly up-regulated in ovalbumin-induced asthma model of mice.

Key words: Asthma, MicroRNA, Inflammatory factor, Cytokine