ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2013, Vol. 5 ›› Issue (1): 12-16.doi: 10.3969/j.issn.1674-3865.2013.01.004

• 实验研究专栏 • 上一篇    下一篇

益气固本胶囊对肾阳虚哮喘模型小鼠脾淋巴细胞增殖功能及相关脏器指数影响的实验研究

陈月园，孙丽平   

  1. 130117 长春，长春中医药大学研究生学院（陈月园）；130021 长春，长春中医药大学附属医院儿科（孙丽平）
  • 出版日期:2013-02-25 发布日期:2019-04-25
  • 通讯作者: 孙丽平，130021 长春，长春中医药大学附属医院儿科。
  • 作者简介:陈月园（1984-），女，长春中医药大学2010级硕士研究生在读。研究方向：小儿呼吸系统、消化系统疾病的中医药防治。
  • 基金资助:
    国家自然科学基金面上项目（30973773）；吉林省科技发展计划项目（200905125）

Experimental research into the influence of Yiqi Guben capsule on the spleen lymphocyte proliferation function and organ index in the kidneyYang deficiency asthma model mice.

CHEN Yueyuan，SUN Liping.   

  1. The Graduate School of Changchun University of Traditional Chinese Medicine，Changchun 130117，China
  • Online:2013-02-25 Published:2019-04-25

PDF (PC)

103

摘要：
目的:观察益气固本胶囊对肾阳虚哮喘模型小鼠脾淋巴细胞增殖功能、脏器指数的影响并探讨其临床意义。
方法:将96只昆明种小鼠随机分成正常对照组、哮喘组、肾阳虚哮喘组、金匮肾气丸组和益气固本胶囊低、中、高剂量组，除哮喘组12只外，其余各组14只，雌雄各半。制备肾阳虚哮喘模型，期间给予受试药物进行干预，记录各组小鼠造模及给药期间，体质量、体温及活动、毛色、饮食、呼吸频率等一般状况变化，实验结束后，取脾脏检测脾淋巴细胞的增殖功能；取肾、脾、胸腺称重，计算其脏器系数。
结果:益气固本胶囊组和金匮肾气丸组小鼠体质量及胸腺质量明显高于肾阳虚哮喘组，差异有统计学意义（P＜0.05）；益气固本胶囊中剂量组、高剂量组及金匮肾气丸组小鼠由脂多糖刺激的脾淋巴细胞增殖明显高于肾阳虚哮喘组，差异有统计学意义（P＜0.05）。
结论:益气固本胶囊能明显改善肾阳虚哮喘小鼠的一般状态；恢复小鼠免疫器官质量；提高脾淋巴细胞的增殖功能。

Abstract:
Objective：To observe the influence of Yiqi Guben capsule on the spleen lymphocyte proliferation function and organ index in the kidneyYang deficiency asthma model mice and to explore its clinical significance.MethodsTotally 96 mice were randomly divided into seven groups: normal control group, asthma group, the kidneyYang deficiency asthma group, kidneyqitonifying pill group, the Yiqi Guben capsule lowdose group, middledose group, highdose group. Apart from the asthma group consisting of 12 mice, the remaining groups each consisted of 14 mice(male and female in half). kidneyYang deficiency asthma groups were treated with Yiqi Guben capsule. Recorded changes in the general condition of the mice in each group during modeling and the period of administration, such as, body weight, body temperature and activity, hair color, diet, breathing frequency, etc. After the experiment, took the spleen and examined the lymphocyte proliferation function; took the kidney, spleen and thymus, and weighed them and calculated the organ coefficient.ReslutsIn the Yiq Guben capsule group and kidneyqitonifying pill group, the mice weight and thymus weight were significantly higher than the kidneyYang deficiency asthma group; in the Yiqi Guben capsule middledose group, the highdose group and kidneyqitonifying pill group, the spleen lymphocyte proliferation by LPS stimulation was significantly higher than the kidneyYang deficiency asthma group.ConclusionYiqi Guben capsule can significantly improve the general state of the kidneyYang deficiency asthma mice, restore immune organ weights, and improve the function of the spleen lymphocyte proliferation.