Abstract: Objective:To investigate the replication method of sodium valproate-induced animal model of autism.Methods:Femalerats received a single intraperitoneal injection of sodium valproate(600 mg/kg) on the 12.5 day after conception in the experiment，the pups as model group;and control females were injected with physiological saline at the same time，the pups as control group. Sodium valproate was dissolved in saline at a concentration of 250 g/L. Observe and test the physical development，social interaction，communication and stereotyped，repetitive patterns of behaviors of the pups. Test physical development and social interaction and communication and physical contact with companion.Results:Compared with control group，model group rats showed lower body weight，delayed open eyes，attenuated coordination of direction and swimming，lower levels of following and push-crawl and higher levels of self-grooming，and lower levels of sniffs of the cotton swab of rat urine.Conclusion:Theoperation of replicate sodium valproate-induced animal model of autism is simple. The success rate of rat model is high.