Abstract: Objective To investigate the characteristics and explore the clinical significance of intestinal flora in asphyxiated neonates.Methods Seven neonates with asphyxia were chosen from Shanghai Children's Hospital as asphyxia group,and 11 healthy neonates were included in the control group. The 16S rRNA sequencing was used to study the difference in intestinal micro-ecology in neonates with asphyxia.Results The diversity index of flora in the neonates with asphyxia was higher, and the dominant bacteria were Clostridium, Veillonella, Lunamonomonas, Anaerobic Sclerotium, and Boschia. Those in the neonates of the control group were Gemini, Streptococcus granulosus, Sarcobacteria, Haemophilus, and Pasteuraceae.Conclusion This study has confirmed that there are significant differences in the intestinal flora between the two groups. Asphyxia may play an important role in the colonization of early intestinal flora in neonatal asphyxia, and may further become a cause of gastrointestinal dysfunction.

Key words:

Neonatal asphyxia, Gastrointestinal microbiome, High-throughput sequencing