ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2013, Vol. 5 ›› Issue (6): 514-517.doi: 10.3969/j.issn.16743865.2013.06.013

• 论著 • 上一篇    下一篇

银翘散精简方、玉屏风散提取物在鸡胚中抗甲型流感病毒FM1株阻断与抑制作用研究

胡楠楠,王雪峰,王子,岳志军,南春红,朱万青,暴春晓   

  1. 110032 沈阳，辽宁中医药大学博士后流动站（胡楠楠）；辽宁中医药大学附属医院儿科(王雪峰)；儿科门诊（王子）；病毒室（岳志军，南春红）；沈阳市儿童医院ICU（朱万青）；辽宁中医药大学（暴春晓）
  • 出版日期:2013-12-25 上线日期:2019-04-25
  • 通讯作者: 王雪峰，110032 沈阳，辽宁中医药大学附属医院儿科。
  • 作者简介:胡楠楠（1982-），女，医学博士，主治医师。研究方向：中医药防治小儿病毒性疾病。
  • 基金资助:
    国家自然科学基金(81072975)

  • Published:2013-12-25 Online:2019-04-25

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摘要：
目的观察银翘散精简方、玉屏风散提取物在鸡胚内抗病毒阻断和抑制的作用。
方法采用鸡胚实验技术观察银翘散精简方、玉屏风散提取物对甲型流感病毒FM1株的阻断和抑制作用。
结果流感病毒鸡胚半数感染量EID50为10-6；银翘散精简方、玉屏风散提取物对鸡胚最大无毒浓度分别为大于39.15、36.2 g/L，利巴韦林最大无毒剂量为100 g/L。银翘散精简方提取物对甲型流感病毒FM1株毒力阻断作用：半数有效量（ED50）为7.41 g/L，治疗指数（TI）大于5.28，浓度为19.58 g/L时鸡胚未感染率为100%，优于其他浓度（P<0.05）；银翘散精简方提取物对甲型流感病毒FM1株毒力抑制作用：ED50为9.91 g/L，TI大于3.95，浓度为39.15 g/L时鸡胚未感染率为100%，优于其他浓度（P<0.05）。玉屏风散提取物对甲型流感病毒FM1株毒力阻断作用：ED50为9.57 g/L，TI大于3.78，浓度为36.2 g/L时鸡胚未感染率为100%，优于其他浓度（P<0.05）；玉屏风散提取物对甲型流感病毒FM1株毒力抑制作用：ED50为10.38 g/L，TI大于3.3，浓度为36.2 g/L和18.1 g/L时（两者毒力抑制作用均等）鸡胚未感染率为83%，优于浓度9.05 g/L（P<0.05）。提取物在鸡胚死亡阻断观察上，在对毒力阻断作用时玉屏风散提取物优于银翘散精简方提取物，在对毒力抑制作用时银翘散精简方提取物优于玉屏风散提取物。各提取物对甲型流感病毒FM1株毒力阻断和抑制作用优于利巴韦林组（P<0.05）。
结论银翘散精简方、玉屏风散提取物对甲型流感病毒FM1株有阻断和抑制作用。

关键词: 银翘散；玉屏风散；鸡胚；甲型流感病毒FM1株；动物, 实验

Abstract:


ObjectiveTo observe the blocking and inhibiting effect of streamlined formula of Yinqiao powder (SFYQP) and Yupingfeng powder (YPFP) extract on influenzaA FM1 strains in chicken embryo.
MethodsChicken embryo experiment technology was used to observe the effectiveness of SFYQP and YPFP extract on inhibiting influenza A virus (IV) FM1 strains.
ResultsED50 of IV infected chicken embryo was 10-6. Themaximum of no toxicity concentration (TC0) of SFYQP and YPFP extract was 39.15 g/L and 36.2 g/L,respectively. The TC0 of ribavirin was 100 g/L. SFYQP extract had effect on preventing infection by IV FM1 strains. The ED50 was 7.41 g/L, and its therapeutic index (TI) was more than 5.28. When its concentration was 19.58 g/L, no infection rate in chicken embryo was 100%, and its effect was better than on other concentration (P＜0.05).SFYQP extract had effect of inhibiting infection by IV FM1 strains. Its ED50 was 9.91 g/L, and its TI was more than 3.95. When its concentration was 39.15 g/L, no infection rate in chicken embryo was 100%, and its effect was better than on other concentration (P＜0.05). YPFP extract also had effect of preventing infection by IV FM1 strains. Its ED50 was 9.57 g/L, and its TI was more than 3.78. When its concentration was 36.2 g/L, no infection rate in chicken embryo was 100%, and its effect was better than on other concentration (P＜0.05).YPFP extract had the effect of inhibiting infection by IV FM1 strains. Its ED50 was 10.38 g/L, and its TI was more than 3.3. When its concentration were 39.15 g/L and 18.1 g/L, no infection rate on two concentrations was both 83%, and their effect was better than on the concentration of 9.05 g/L (P＜0.05). On observing the death blocking of  extract on chicken embryos, the blocking of YPFP extract was more effective than SFYQP extract, though, inhibition of SFYQP extract was more effective than YPFP extract. Moreover,blocking and inhibition of both extracts were obviously better than ribavirin (P＜0.05).
ConclusionSFYQP and YPFP extracts have the effect of blocking and inhibition on IV FM1 strains infected chicken embryo.

Key words: Yinqiao powder (SFYQP) ；Yupingfeng powder (YPFP)；chicken embryo；influenzaA virus (IV) FM1 strains, animal,experiment