ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2019, Vol. 11 ›› Issue (3): 202-205.doi: 10.3969/j.issn.1674-3865.2019.03.006

• 实验论著 • 上一篇    下一篇

益气解毒化瘀小复方及其单味药对大鼠肾小球系膜细胞增殖及凋亡影响的研究

王圣治，陈思敏，刘英楠，郑艳   

  1. 110032 沈阳，辽宁中医药大学附属医院肾内科（王圣治）；110847 沈阳，辽宁中医药大学2015级中医内科学专业研究生（陈思敏，刘英楠），2016级中医内科学专业研究生（郑艳）
  • 出版日期:2019-06-25 发布日期:2019-07-04
  • 通讯作者: 王圣治，E-mail：wszln@163.com
  • 作者简介:王圣治（1972-），男，医学博士，副主任医师，硕士研究生导师。研究方向：中西医治疗肾脏病的临床及实验研究
  • 基金资助:
    2015年中医药行业科研专项（201507001-03）

Effects of Yiqi Jiedu Huayu Xiao Fufang and its single drug on proliferation and apoptosis of glomerular mesangial cells in rats

WANG Shengzhi, CHEN Simin，LIU Yingnan，ZHENG Yan   

  1. Affiliated Hospital of Liaoning University of Traditional Chinese Medicine，Shenyang 110032，China
  • Online:2019-06-25 Published:2019-07-04

PDF (PC)

91

摘要： 目的
观察益气解毒化瘀小复方及其单味药各组对大鼠肾小球系膜细胞增殖、生长抑制及细胞凋亡的影响并探究其机制。
方法
60只Wistar大鼠适应性饲养1周后，随机分为正常对照组、小复方组、黄芪组、丹参组、白花蛇舌草组、替米沙坦组，每组10只；正常对照组每日灌服蒸馏水4 mL，各药物组分别早晚各一次灌服药液2 mL，连续3 d。常规培养大鼠肾小球系膜细胞，将培养后的肾小球系膜细胞随机分为正常对照组、小复方组、黄芪组、丹参组、白花蛇舌草组、替米沙坦组、血管紧张素Ⅱ组。各组均加入血管紧张素Ⅱ 1×10-6mol/L。采用MTT比色法检测各组对大鼠系膜细胞增殖作用情况，流式细胞仪测定各组对大鼠肾小球系膜细胞凋亡作用情况。
结果
与血管紧张素Ⅱ组比较，各药物组对血管紧张素Ⅱ诱导的系膜细胞增殖均有抑制作用，均能够增加系膜细胞凋亡率差异有统计学意义(P<0.01)；各药物组与小复方组比较，小复方组抑制肾小球系膜细胞增殖作用和凋亡作用最显著，差异有统计学意义(P<0.01或0.05)；其余各组之间比较差异无统计学意义（P>0.05）。
结论益气解毒化瘀小复方及其单味药对大鼠肾小球系膜细胞增殖及凋亡均有抑制作用，从而能够减缓肾小球硬化发展进程，其中益气解毒化瘀小复方作用最强。

关键词: 系膜细胞, 益气解毒化瘀, 细胞增殖, 细胞凋亡

Abstract: Objective
To observe the effect of Yiqi Jiedu Huayu Xiao Fufang and its single drug on proliferation and apoptosis of glomerular mesangial cells in rats and explore its mechanism.
Methods
A total of 60 Wistar rats were randomly divided into normal group, Xiaofufang group, Huangqi group, Danshen group, Baihuasheshecao group and Telmisartan group, with 10 rats in each group. The normal group was given 4 mL of distilled water daily, and each drug group was given 2 mL of drug in the morning and evening for 3 consecutive days. Rat mesangial cells were routinely cultured, and the cultured mesangial cells were randomly divided into normal group, Xiaofufang group, Huangqi group, Danshen group, Baihuasheshecao group, Telmisartan group, and Angiotensin Ⅱ group. Angiotensin Ⅱ 1×10-6mol/L was added to each group. The proliferation of rat mesangial cells was detected by MTT colorimetric assay. The apoptosis of rat mesangial cells was determined by flow cytometry.
Results
Compared with Angiotensin II group, other drug intervention groups had effect of inhibiting the proliferation and increasing apoptosis rate of mesangial cells(P<0.01 or P<0.05); there was no significant difference among the other groups(P>0.05). Compared with other drug intervention groups, the Xiaofufang group had the strongest inhibition and apoptosis of mesangial cells(P<0.01 or P<0.05); there was no significant difference among the other groups(P>0.05).
Conclusion
Yiqi Jiedu Huayu Xiao Fufang and its single drug can inhibit the proliferation and apoptosis of rat mesangial cells, which can slow the development of glomerular sclerosis, and the effect of Yiqi Jiedu Huayu Xiao Fufang is the best.

Key words: Mesangial cells, Yiqi Jiedu Huayu Xiao Fufang, Cell proliferation, Apoptosis