ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2013, Vol. 5 ›› Issue (1): 5-8.doi: 10.3969/j.issn.1674-3865.2013.01.002

• 实验研究专栏 • 上一篇    下一篇

益气固本胶囊调节肺气虚小鼠脾淋巴细胞增殖作用的实验研究

孙莹莹，孙丽平   

  1. 作者单位：130117 长春，长春中医药大学研究生学院（孙莹莹）；130021 长春，长春中医药大学附属医院儿科（孙丽平）
  • 出版日期:2013-02-25 发布日期:2019-04-25
  • 通讯作者: 通讯作者：孙丽平，130021 长春，长春中医药大学附属医院儿科。
  • 作者简介:孙莹莹（1987-），女，长春中医药大学2010级硕士研究生在读。研究方向：小儿呼吸系统、消化系统疾病的中医药防治。
  • 基金资助:
    国家自然科学基金面上项目（30973773）；吉林省科技发展计划项目（200905125）

The experimental study of Yiqi Guben capsule in adjusting the splenic lymphocyte proliferation of lungQi deficiency mice

SUN Yingying，SUN Liping.   

  1. The Graduate School of Changchun University of Traditional Chinese Medicine，Changchun 130117，China
  • Online:2013-02-25 Published:2019-04-25

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摘要：
目的:探讨益气固本胶囊对肺气虚小鼠脾淋巴细胞增殖功能的调节作用。
方法:将96只小鼠（雌雄各半）随机分为正常对照组、模型组、益气固本胶囊高、中、低剂量组、玉屏风散颗粒组6组，每组16只。除正常对照组外，其余各组小鼠均采用改良烟熏法制备肺气虚证模型，造模后灌胃给予相应的受试药物5周，采用XTT法检测由脂多糖或ConA刺激的小鼠脾淋巴细胞的增殖功能。
结果:小鼠造成肺气虚模型后其脾B、T淋巴细胞增殖功能均明显低于正常对照组，差异有统计学意义(P<0.001)；而灌胃给予受试药物后，脾B、T淋巴细胞增殖功能均明显升高，与模型组比较，玉屏风散颗粒组、益气固本胶囊中、高剂量组的脾淋巴细胞增殖功能均明显升高，差异均有统计学意义(P<0.05)，其中益气固本胶囊高剂量组小鼠脾淋巴细胞的增殖功能最强。
结论:益气固本胶囊可提高肺气虚小鼠脾淋巴细胞增殖活性，从而调节其免疫功能。

Abstract:
Objective：To discuss the Yiqi Guben capsule role in regulating the splenic lymphocyte proliferation of lungQi deficiency mice.
Methods：Totally 96 mice (male and female in half) were equally divided into 6 groups: normal control group, model group, Yiqi Guben capsules highdose group, middledose group and lowdose group, control group of Yupingfeng powder. Apart from the normal group, the mice in the rest of each group were made into lungQi deficiency syndrome models by adopting improved smoked method; tested drugs were given by intragastric administration for five weeks; XTT method was taken to assay the proliferation of splenic lymphocyte stimulated by the LSP or ConA.
Results：After lungQi deficiency modeling，the function of the splenic B and T lymphocyte proliferation was significantly lower than the normal control group (P<0.001)；after the tested drugs were given by intragastric administration, the function of splenic B and T lymphocyte proliferation was significantly increased; compared  with the model group, the splenic lymphocyte proliferation function of control group, of Yupingfeng powder group, of Yiqi Guben capsule highdose group and middledose group was significantly increased(P<0.05)，and the increase of Yiqi Guben capsule highdose group was the most significant.
Conclusions：Yiqi Guben capsule can improve the lymphocyte proliferation activity of lungQi deficiency mice，thereby regulate the immune function.