ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2014, Vol. 6 ›› Issue (4): 315-318.doi: 10.3969/j.issn.1674-3865.2014.04.013

• 综述 • 上一篇    下一篇

甲型流感病毒FM1株诱导感染不同鼠龄小鼠肺炎模型比较

胡楠楠，王雪峰，岳志军，南春红，朱万青，暴春晓   

  1. 110032 沈阳，辽宁中医药大学博士后流动站（胡楠楠）；辽宁中医药大学附属医院儿科（王雪峰），病毒室（岳志军，南春红）；沈阳市儿童医院ICU（朱万青）；辽宁中医药大学研究生院（暴春晓）
  • 收稿日期:2014-04-13 出版日期:2014-08-25 上线日期:2014-08-25
  • 通讯作者: 王雪峰
  • 作者简介:胡楠楠（1982-），女，辽宁中医药大学博士后在研，主治医师。研究方向：中医药防治小儿病毒性疾病。
  • 基金资助:
    国家自然科学基金资助（81273800）

Comparison of pneumonia mouse models of different ages with infection induced by influenza A virus FM1 strains

HU Nan nan，WANG Xue feng,YUE Zhi jun，NAN Chun hong，ZHU Wan qing，BAO Chun xiao   

  1. Liaoning University of Traditional Chinese Medicine Postdoctoral flow station,Shenyang 110032，China.
  • Received:2014-04-13 Published:2014-08-25 Online:2014-08-25

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摘要： 目的:评价昆明种小鼠哪一鼠龄段为甲型流感病毒FM1株最佳模型。方法:150只清洁级昆明种小鼠，雌雄各半，鼠龄均等，分为幼龄模型组（40只）、成龄模型组（40只）、老龄模型组（40只）、幼龄正常组（10只）、成龄正常组（10只）、老龄正常组（10只），观察小鼠状态，分别于感染3、5、7 d，检测其肺湿质量、肺组织肉眼病理评分、肺组织病理切片以及肺组织FQ-PCR病毒载量。结果:幼龄模型组14 d内死亡最多，整体状态最差，肺部病变最为严重，肺组织病毒载量呈递增趋势。成龄模型组14 d内死亡最少，整体状态最好，肺部病变少，肺组织病毒载量无复制的现象。老龄模型组整体状态在第3天出现肺组织病变，但之后有恢复的趋势，第5天后，病毒载量呈下降趋势。结论:幼龄昆明种小鼠为甲型流感病毒最佳理想模型。

关键词: 肺炎, 流感病毒, 动物, 实验, 小鼠

Abstract: Objective:To evaluate which rat age segment of Kunming mice is the best model of influenza A virus FM1 strains. Methods:A total 150 Kunming mice of clean grade, half male and half female, with equal age, were randomly divided into young model group (40 rats), mature model group (40 rats), aged model group (40 rats), young normal group (10 rats), mature normal group (10 rats), and aged normal group (10 rats). The mice were observed, and respectively at the end of 3,5 and 7 days of infection were detected lung wet weight;lung tissue pathology score with naked eyes, lung histopathology and lung tissue FQ-PCR viral load testing were also performed.Results:In the young model group, the highest death occurred in 14 days, the overall state was the worst with most severe lung disease, and viral load in lung tissue showed an increasing trend. In mature model group, the death was the least in 14 days, the overall state was the best, basically there was no lung disease, and lung tissue viral load had no copying phenomenon. In aged rats, the overall state showed lung tissue lesions in the first three days, but there was a tendency to recover and after 5 days, viral load tended to decline.Conclusion:The young mice are the idealest model of influenza A virus.

Key words: pneumonia；influenza virus；animal, experiment；mouse