ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2015, Vol. 7 ›› Issue (4): 316-319.doi: 10.3969/j.issn.1674-3865.2015.04.007

• 实验论著 • 上一篇    下一篇

蝎黄解痉治哮颗粒对哮喘小鼠模型中p38MAPK和Th1/Th2类细胞因子变化的影响

冯晓纯，段晓征，朱浩宇，延光海，郑美珠   

  1. 130021 长春，长春中医药大学附属医院儿科（冯晓纯，段晓征，朱浩宇）；133002 吉林 延吉，延边大学医学院解剖教研室（延光海）；130021 长春，长春中医药大学研究生院（郑美珠）
  • 收稿日期:2015-04-10 出版日期:2015-08-25 上线日期:2015-08-25
  • 通讯作者: 冯晓纯
  • 作者简介:冯晓纯（1960-），女，医学硕士，教授、主任医师，博士研究生导师。研究方向：小儿肺系疾病的中西医结合治疗。
  • 基金资助:
    吉林省科技发展计划项目（20130102068JC）

Effect of scorpion ephedra antispasmodic granules for asthma on p38MAPK and Th1/Th2 cytokines in a mouse model of asthma

FENG Xiaochun， DUAN Xiaozheng，ZHU Haoyu，YAN Guanghai，ZHENG Meizh   

  1. Department of Pediatrics, Affiliated Hospital of Changchun University of Traditional Chinese Medicine，Changchun 130021，China
  • Received:2015-04-10 Published:2015-08-25 Online:2015-08-25
  • Contact: FENG Xiaochun

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摘要： 目的 探讨蝎黄解痉治哮颗粒对哮喘小鼠气道炎症和Th1/Th2类细胞因子变化的影响及其可能机制。方法 将24只6～8周龄雄性小鼠随机分为正常对照组、哮喘模型组、小剂量中药组、大剂量中药组，每组6只。制备支气管哮喘模型，小剂量中药组、大剂量中药组分别给予蝎黄解痉治哮颗粒免煎制剂每日0.72、2.89 g，按20 mL/kg配制成水混匀溶液灌胃每日2次，连续10 d；正常对照组和哮喘模型组小鼠给予等量生理盐水。分别采用酶联免疫吸附法和免疫印迹法检测支气管肺泡灌洗液（BALF）中白细胞介素4（IL-4）、IL-5、γ干扰素（IFN-γ）含量以及肺组织中磷酸化p38丝裂原活化蛋白激酶（p38MAPK）的变化，并观察BALF中炎症细胞和肺组织病理学改变。结果 哮喘模型组小鼠BALF中炎症细胞计数、IL-4、IL-5含量水平升高和IFN-γ水平降低；肺组织中磷酸化p38MAPK表达水平升高，与正常对照组比较，差异有统计学意义（P＜0.05）。小、大剂量中药组小鼠BALF中炎症细胞计数、IL-4、IL-5含量水平下降和IFN-γ水平升高；肺组织中磷酸化p38MAPK表达水平降低，与哮喘模型组比较，差异均有统计学意义（P＜0.05）。小、大剂量中药组比较，差异无统计学意义（P＞0.05）。结论 p38MAPK可能参与了支气管哮喘的发病过程。蝎黄解痉治哮颗粒对哮喘小鼠具有保护作用，其机制可能与抑制p38MAPK磷酸化、调节Th1/Th2平衡失调、减轻炎症细胞浸润有关。

关键词: 哮喘, 蝎黄解痉治哮颗粒, p38MAPK, Th1/Th2细胞因子, 小鼠, 动物, 实验

Abstract: Objective To explore the effect of scorpion ephedra antispasmodic granules for asthma on airway inflammation in asthmatic mice and Th1/Th2 type cytokines changes and its possible mechanism.Methods Totally 24 male mice of only 6 to 8 weeks old were randomly divided into four groups:normal control group, asthma model group, small-dose traditional Chinese medicine group, large-dose Chinese medicine group, 6 mice in each group. Make bronchial asthma model, and the samll-dose traditional Chinese medicine group and large-dose TCM group were given scorpion ephedra antispasmodic granules for asthma to let boiled preparation, 0.72 and 2.89 g daily to make 20 mL/kg aqueous mix solution for stomach irrigation twice a day, for 10 days; normal control group and the asthma model group were given the equal amount of saline. Enzyme linked immune sorbent assay and immunoblotting were used to detect leukocyte mediated-4(IL-4), IL-5, interferon gamma(IFN-γ) content in bronchial alveolar irrigation lavage fluid (BALF) and phosphorylated p38 mitogen activated protein kinase(p38MAPK) changes in the lung tissue, and the pathological changes of inflammatory cells in BALF and lung tissue were observed.Results In BALF of asthmatic model group,inflammatory cell count, IL-4 and IL-5 levels increased and IFN-γlevels decreased; in lung tissues phosphorylated p38MAPK expression level increased, and compared with the normal control group, the difference was statistically significant(P<0.05). The inflammatory cell counts, IL-4 and IL-5 levels decreased and IFN-γ levels increased, in BALF of large-dose Chinese medicine group increased; in lung tissues the phosphorylated p38MAPK expression level reduced, and compared with the asthma model group, the differences were statistically significant(P<0.05). Comparing small with large-dose TCM group, the difference was not statistically significant(P>0.05). Conclusion p38MAPK may be involved in the pathogenesis of bronchial asthma. Scorpion ephedra antispasmodic granules for asthma has a protective effect on asthma in mice, and the mechanism may be related to inhibiting p38MAPK phosphorylation, regulating the imbalance of Th1/Th2 cells and reducing the inflammatory cell infiltration.

Key words: asthma, scorpion ephedra antispasmodic granules for asthma, p38MAPK, Th1/Th2 cytokine, mouse；animal, experiment