关键词: 抽动障碍, 柔肝宁静汤, 纹状体, 多巴胺, 多巴胺转运蛋白, 大鼠

Abstract: Objective:To observe the effects of Rougan Ningjing decoction on the transportation and metabolism of dopamine in the striatum and the behavior of rats with tic disorders.Methods:Twenty male SD rats were randomly divided into 4 groups:the control group was normally fed and intraperitoneally injected with normal saline for 1 week;the model group,the traditional Chinese medicine group and the western medicine group were intraperitoneally injected with iminodipropionitrile solution for 1 week and simultaneously subjected to chronic restraint suspension for 3 weeks.After successful modeling,the traditional Chinese medicine group was given Rougan Ningjing decoction granule solution,the western medicine group was given tiapride hydrochloride suspension,and the control group and the model group were given normal saline,with a daily dose of 10 mL/kg for 3 weeks.After the intervention for each group,stereotyped movement scores and behavioral experiments were conducted in each group;the changes in dopamine and homovanillic acid levels in the striatum of rats in each group were determined by enzyme linked immunosorbent assay;the expression of dopamine transporter in the striatum was determined by Western blot.Results:(1) The stereotyped movement score of the model group was significantly higher than that of the control group,and the stereotyped movement scores of the traditional Chinese medicine group and the western medicine group were lower than those of the model group,with statistically significant differences(P<0.05).(2) In the open field test,the movement speed,total distance and time spent in the central area of rats in the control group,the traditional Chinese medicine group and the western medicine group were lower than those in the model group,with statistically significant differences(P<0.05);in the elevated plus maze test,the number of entries into the open arms and the time spent in the open arms of rats in the control group,the traditional Chinese medicine group and the western medicine group were higher than those in the model group,but the differences were not statistically significant(P>0.05).(3) The content of dopamine and the expression of dopamine transporter in the striatum of rats in the model group were lower than those in the control group,and the content of dopamine and the expression of dopamine transporter in the striatum of rats in the traditional Chinese medicine group and the western medicine group were higher than those in the model group,with statistically significant differences(P<0.05).The content of homovanillic acid in the striatum of rats in the model group was higher than that in the control group,the traditional Chinese medicine group and the western medicine group,but the differences were not statistically significant(P>0.05).Conclusion:Rougan Ningjing decoction can increase the content of dopamine in the striatum of rats with tic disorders,improve emotional disorders,and relieve tic symptoms.

Key words: Tic disorders, Rougan Ningjing decoction, Striatum, Dopamine, Dopamine transporter；Rat