ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2011, Vol. 3 ›› Issue (1): 23-26.doi: 10.3969/j.issn.1674-3865.2011.01.012

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中药二步序贯治疗对哮喘小鼠气道组织核因子κB系统的影响

杨召川，陈蕾，刘秀美，常红，曲政海   

  1. 266003 山东 青岛，青岛大学医学院研究生部（杨召川）；青岛大学医学院附属医院儿科（陈蕾，刘秀美，常红，曲政海）
  • 收稿日期:2010-11-09 出版日期:2011-02-25 上线日期:2011-02-25
  • 通讯作者: 曲政海
  • 作者简介:杨召川（1984-），女，2008级青岛大学医学院硕士研究生。研究方向：小儿呼吸系统及变态反应性疾病的基础研究。
  • 基金资助:
    山东省自然科学基金资助课题(Y2007C113)

The effects of two-step sequential therapy on signal pathway of NF-κB on airway inflammation in asthmatic mice

YANG Zhao chuan,CHEN Lei,LIU Xiu mei   

  1. Department of Pediatrics，the Affiliated Hospital of Medical College of Qingdao University，Qingdao 266003，China
  • Received:2010-11-09 Published:2011-02-25 Online:2011-02-25

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摘要： 目的：探讨中药二步序贯治疗对哮喘小鼠气道组织核因子κB（NF-κB)）系统的影响。方法：健康雌性昆明系小鼠40只随机分为正常对照组、模型对照组、布地奈德组和二步序贯组，每组10只。采用卵清白蛋白致敏建立哮喘小鼠模型，并分别予以布地奈德和二步序贯干预治疗。观察哮喘小鼠气道组织病理变化，气道组织NF-κB、GATA结合蛋白3(GATA-3)以及肺泡灌洗液白细胞介素4（IL-4）的变化。结果：布地奈德组和二步序贯组气道组织炎性改变较模型对照组明显减轻，较正常对照组明显加重，差异均有统计学意义（P＜0.01）；布地奈德组和二步序贯组气道炎症改变差异无统计学意义（P＞0.05）。布地奈德组和二步序贯组气道组织NF-κB、GATA-3、IL-4表达水平较模型对照组均降低，较正常对照组均升高，差异均有统计学意义（P<0.01）；布地奈德组和二步序贯组比较，差异无统计学意义（P＞0.05）。结论：二步序贯治疗可能通过抑制NF-κB信号转导通路活性，进而降低GATA-3的表达，降低Th2类细胞因子，减轻哮喘小鼠气道炎症。

关键词: 哮喘；人参五味子汤；小青龙汤；核因子κB；GATA-3；白细胞介素4；小鼠；动物, 实验

Abstract: Objective：To study the effects of two-step sequential therapy on signal pathway of NF-κB of airway inflammation in asthmatic mice.Methods：Forty healthy female Kunming mice were divided into four groups randomly(n=10,in each group):normal control group,asthma group,budesonide group and two-step sequential therapy group.The asthmatic models were established by ovalbumin,and budesonide and two-step sequential therapy were applied to them.Then the pathologic changes in airway tissues and bronchoalveolar lavage fluid(BALF) in cytology were observed.The level of nuclear factor-κB(NF-κB) and GATA-3 in airway tissues was detected by immunohistochemical method.The level of interleukin-4（IL-4） in BALF was detected by enzyme-linked immunosorbent assay(ELISA).Results：The treatment groups had less inflammatory cell infiltration than asthmatic group(P<0.01),and more than normal control group(P<0.01).There was no significant difference between the budesonide group and two-step sequential therapy group(P>0.05).The level of NF-κB,GATA-3 and IL-4 decreased in the budesonide group and two-step sequential therapy group compared with the asthmatic group(P<0.01),but increased compared with the normal control group(P<0.01).There was no significant difference between budesonide group and twostep sequential therapy group(P>0.05).Conclusions：The two-step sequential therapy may inhibit the activity of the signal transduction pathway of NF-κB,which can reduce the expression of GATA-3 and class cytokines of Th2,hence plays an important role in treating asthmatic mice．

Key words: Asthmatic；Ginseng-schisandrae decoction;Minor green-blue dragon decoction;Nuclear factor-κB;GATA-3;Interleukin4；Mice；Animal, experiment