关键词: 肺炎, 病毒性, 呼吸道合胞体病毒, 干扰素类Ⅰ型, 兔

Abstract: ObjectiveTo study the effect of Jinxin oral liquid on expressions of mRNA and protein of interferonβ(IFNβ) in RSVinfected human embryonic lung fibroblast cells in vitro and to discuss the treatment mechanism of Jinxin oral liquid to the RSV infected cell proliferation. MethodsWe successfully established the cell model of RSV by the long strain（A type of RSV).We divided them into five groups，which were normal cell group,RSV group,blank serum group,ribavirin group and Jinxin oral liquid group.In the experiments,the real time PCR and ELISA were used to examine expressions of mRNA and protein of IFNβ in RSVinfected human embryonic lung fibroblast cells in different groups. ResultsThe mRNA of IFNβ expression in human embryonic lung fibroblast cells did not significantly descend after being infected by RSV(the value of 2△△Ct equaled 1) compared with those in normal fibroblast cells(the value of 2△△Ct equaled 1.79)（P>0.05）.The relative value of blank serum group and ribavirin group was 0.967 and 0.86（P>0.05）.In the relative value of Jinxin oral liquid group，it was 0.57（P>0.05）. Three prescription methods were adopted.First method was that the cells were added the medicine serum and then they were infected by RSV.The cells were infected by RSV and then were added the medicine serum in the second method.In the last method cells were interfered with the medicine serum alone.Unfortunately，the protein expression of IFNβ in each group was 0 ng/L. Conclusion（1）RSV does not stimulate human embryonic lung fibroblast cells to express the mRNA and protein of IFNβ.（2) Jinxin oral liquid does not induce the mRNA and protein of IFNβ production.Jinxin oral liquid might inhibit the RSV infected cells proliferation.However,there might be other ways independent of IFNβ.

Key words: Pneumonia, virus；Respiratory syncytial viruses;Interferon type Ⅰ;Rabbits