五虎汤对呼吸道合胞病毒诱导的外泌体引起哮喘小鼠气道炎症的干预作用研究

doi:10.20274/j.cnki.issn.1674-3865.2025.05.008

Abstract

Abstract:

Objective To investigate the effects of Wuhu decoction on airway inflammation-related indicators in asthmatic mice induced by respiratory syncytial virus (RSV)-primed bone marrow mesenchymal stem cell-derived exosomes(BMSCs-Exo-RSV). Methods The asthma mouse model was established by intranasal administration of BMSCs-Exo-RSV. Sixty SPF-grade male C57 mice were randomly divided into six groups(n=10 per group): control group, BMSCs-Exo-RSV model group, ribavirin intervention group, and low-, medium-, and high-dose Wuhu decoction intervention groups. Interventions were performed and recorded once daily for two weeks. After the experiment, the behaviors of the mice in each group were compared. Pulmonary function testing using the DSI Buxco PFT system was conducted to measure residual volume/total lung capacity ratio(RV/TLC) and airflow limitation of the pulmonary function of mice; histopathological changes of lung tissues were compared via Masson and HE staining;ELISA quantification of IFN-γ, IL-12, IL-4, and IL-6 levels in bronchoalveolar lavage fluid(BALF) was performed; qPCR was used to determine microRNA-144(miRNA-144) expression. Results Compared with the control group, BMSCs-Exo-RSV model mice exhibited clinical symptoms(tachypnea, nasal scratching, sneezing, weight loss), elevated RV/TLC(P<0.01) that indicated airflow limitation, aggravated inflammatory injury of lung tissues by pathological examination, decreased IFN-γ and IL-12(P<0.01) and increased IL-4 and IL-6(P<0.01) in BALF that further suggested formation of airway inflammation, and upregulated miRNA-144(P<0.01). Compared with model group, Wuhu decoction(all doses) and ribavirin groups showed improvement in clinical symptoms of tachypnea, nasal scratching, sneezing and weight loss, airflow limitation was reduced(P<0.01), pulmonary inflammation was improved, IFN-γ and IL-12(P<0.01) increased while IL-4 and IL-6 decreased(P<0.01) in BALF, and the expression of miRNA-144 decreased(P<0.01). Conclusion Wuhu decoction effectively modulates immune balance and alleviates airway inflammation in BMSCs-Exo-RSV-induced asthmatic mice, which may be related to its inhibition of miRNA-144 upregulation.

Key words: Asthma, Airway inflammation, Wuhu decoction, Exosomes, Bone marrow mesenchymal stem cells(BMSCs), miRNA, Mice

CLC Number:

R725.6

Zhi ZHOU, Mengqing WANG, Yinhe LUO, Bing YAO, Jinglei LIU, Guohong WANG, Xueyi LI, Xiao MA. Investigating the therapeutic role of Wuhu decoction for airway inflammation in mice with asthma caused by RSV-induced exosomes[J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2025, 17(5): 402-409.

Figures/Tables 6

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References 59

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组别	n	体质量
空白组	10	24.78±1.27
模型组	10	20.52±1.32^a
利巴韦林干预组	10	22.84±0.65^b
五虎汤低剂量干预组	10	22.88±0.89^b
五虎汤中剂量干预组	10	23.16±1.10^b
五虎汤高剂量干预组	10	22.69±1.16^b
F值		15.470
P值		<0.01

组别	n	RV/TLC
空白组	10	0.033±0.006
模型组	10	0.106±0.019^a
利巴韦林干预组	10	0.037±0.014^b
五虎汤低剂量干预组	10	0.057±0.017^b
五虎汤中剂量干预组	10	0.049±0.010^b
五虎汤高剂量干预组	10	0.054±0.016^b
F值		34.030
P值		<0.01

组别	n	IFN-γ	IL-12	IL-4	IL-6
空白组	10	502.41±13.74	7.61±0.24	116.50±7.23	84.19±8.14
模型组	10	276.65±19.93^a	4.17±0.27^a	225.58±11.75^a	258.74±18.98^a
利巴韦林干预组	10	391.42±15.21^b	5.28±0.33^b	168.34±5.50^b	153.49±7.32^b
五虎汤低剂量干预组	10	381.98±15.56^b	4.90±0.21^b	174.93±5.27^b	145.70±6.18^b
五虎汤中剂量干预组	10	403.22±16.77^b	5.29±0.28^b	163.46±6.68^b	141.02±5.35^b
五虎汤高剂量干预组	10	391.77±18.43^b	5.20±0.36^b	169.78±9.83^b	146.47±11.44^b
F值		185.267	155.822	192.752	286.677
P值		<0.01	<0.01	<0.01	<0.01

组别	n	miRNA-144
空白组	10	3.09±0.68
模型组	10	41.42±4.63^a
利巴韦林干预组	10	19.61±3.31^b
五虎汤低剂量干预组	10	19.73±2.52^b
五虎汤中剂量干预组	10	16.77±0.96^b
五虎汤高剂量干预组	10	18.33±1.56^b
F值		215.451
P值		<0.01

miRNA-144	IFN-γ	IL-12	IL-4	IL-6
r值	-0.987	-0.872	0.988	0.997
P值	<0.001	0.024	<0.001	<0.001

Investigating the therapeutic role of Wuhu decoction for airway inflammation in mice with asthma caused by RSV-induced exosomes

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