Abstract: Objective :To investigate the effects of Fangxiaoyin Decoction on the serum concentration of Interleukin10(IL10) and eosinophil of bronchoalveolar lavage fluid in asthmatic mice after early interference treatment.〖WTHZ〗Methods:〖WTBZ〗Sixty healthy Kunming mice were randomly divided into six groups: Group A:normal control group,Group B:asthmatic model group,Group C:low-dose Fangxiaoyin decoction treatment group,Group D:medium-dose Fangxiaoyin decoction treatment group,Group E:high-dose Fangxiaoyin decoction treatment group,Group F:Budesonide(BUD)treatment group.The asthmatic mice model was stimulated with ovalumin.Fangxiaoyin decoction treatment groups were given from the first day sensitization by low-dose (containing crude drug 0.5 g/mL),medium dose (containing crude drug 1.5 g/mL),high-dose (containing crude drug 2.5 g/mL),once a day,each 0.4 mL,for a total of 27 days.At the 24 h after the final stimulation (28 days),the mice were executed.Detect IL10 by ELISA.And then,detect the percentage of EOS of BALF by microscope.〖WTHZ〗Results:〖WTBZ〗The percentage of EOS of BALF in group B was significantly higher than group A(P<0.01).The concentration of IL10 in group B was significantly lower than group A(P<0.01).Group C,D and E the concentrations of IL10 were significantly increased.There was significant difference between groups C,D,E and B(P<0.01) on the concentrations of IL10,and there was no significant difference between groupC,D,E and F(P>0.05).In group C,D,E and F EOS% of BALFwas decreased(P<0.01),there being no significant difference among the groups(P>0.05).〖WTHZ〗Conclusion:〖WTBZ〗Pathological state of asthmatic mice can be greatly improved after early interference treatment by Fangxiaoyin Decoction and BUD.The mechanism may be to increase IL10 and decrease EOS% of BALF.

Key words: Fangxiaoyin Decoction;Asthma;Interleukin10;Mice, inbred strain;Animal,experiment