Abstract:
Objective：To investigate the effect of IGF1 on renal interstitial fibrosis in rats with unilatreal ureteral obstruction(UUO).
Methods：Totally 30 rats were randomly divided into 3 groups, with 10 rats in each group, namely, sham group, model group and IGF1 group. Renal interstitial fibrosis models were made by unilateral ureteral ligation. In the sham group, the abdomen was opened and then stitched; in the model group, physiological saline was intragastrically injected, 0.5 mL a day; in IGF1 group, recombinant human IGF1 was intragastrically injected, 750 mg/(kg·d). At the 14th day after the operation, the rats were anesthetized, and related materials were taken, the kidney function was tested, and   ColⅢ，TGFβ1 and PAI1 expressions in kidneys were observed.
Results：The expression of BUN，Scr，ColⅢ，TGFβ and PAI1 in the model group were significantly higher than those in sham group, the difference having statistic significance. After the intervention of IGF1, the level of BUN，Scr，ColⅢ，TGFβ and PAI1 in IGF1 group was significantly higher than that in sham and model group, the difference had statistic significance（P＜0.05）.
Conclusions：IGF1 plays a role in the pathophysiological process of obstructive nephropathy，especially in the renal interstitial fibrosis.