Abstract:
Objective
To investigate the role of S100 A8/A9 protein in the development of Kawasaki disease and the occurrence of coronary artery lesions.
Methods
A total of 46 children with Kawasaki disease were included as the research subjects, who were treated in Wuxi Children's Hospital from Jan. 2010 to Dec. 2012,and they were divided into two groups based on whether there was coronary artery lesion or not: 20 in the with-lesion group(A) and 26 in the without-lesion group(B). Another 40 children receiving operation for inguinal hernia were chosen as the control group. Peripheral venous blood was collected; the monocytes were separated by stratified fluid density gradient centrifugation of lymphocytes; RNA was extracted; expression of S100 A8/A9 was detected by RT-PCR; the dimer level of plasma S100 A8/A9 was determined by ELISA.
Results
Before the venous injection of immune globulin for children with acute Kawasaki disease, the dimer level of S100 A8/A9 protein in group A and group B was significantly higher than that in control group, the difference being statistically significant(P<0.05),while after the injection, the expression of S100 A8/A9 mRNA was significantly higher than that in control group, and there was statistical difference(P<0.05);the dimer level of S100 A8/A9 protein and S100 A8/A9 mRNA expression in group A were higher than those in group B, with statistical difference(P<0.05).
Conclusion
Before venous injection of immune globulin, the S100 A8/A9 mRNA expression and dimer level is significantly higher than the control group, which suggests that S100 A8/A9 may be involved in the pathogenesis of Kawasaki disease.

Key words: Kawasaki disease, Monocytes, S100 A8, S100 A9, Child