TBX1基因可能通过调控Tgfb2基因的表达参与心脏发育及先天性心脏病的发生

doi:10.3969/j.issn.1674-3865.2018.01.010

Abstract

Abstract:
Objective
By detecting the expression of transforming growth factor beta2 gene (Tgfb2) after TBX1 gene interference, we explored the possible way of TBX1 gene involving in cardiac development and development of congenital heart disease.
Methods
According to the different treatment factors, the cells were divided into NC-siRNA group and TBX1-siRNA group. TBX1-siRNA was transfected in rat embryonic cardiomyocyte H9C2, and the interference efficiency was detected by real-time quantitative PCR (qRT-PCR). The H9C2 cell line at 48h after transfection with TBX1-siRNA for was collected to extract the total RNA, and qRT-PCR method was used to detect the expression level of Tgfb2 gene mRNA after interference.
Results
(1)qRT-PCR was used to detect the expression of TBX1 mRNA in myocardial cells. The expression was (0.22±0.02) at 48h after transfection with TBX1-siRNA. Compared with NC-siRNA (0.99±0.02), the expression of TBX1 mRNA was significantly down-regulated, and the difference was statistically significant(P<0.05). (2)The expression of Tgfb2 mRNA in myocardial cells was detected by qRT-PCR, and the expression of H9C2 cells was transfected with TBX1-siRNA. After 48h, the expression of Tgfb2 mRNA was significantly up-regulated (2.19±0.01) compared with that of NC-siRNA(1.00±0.01). The difference was statistically significant(P<0.05).
Conclusion
The down-regulation of TBX1 gene expression may be involved in the development of heart and the development of congenital heart disease by regulating the expression of Tgfb2 gene.

Key words: Congenital heart disease, TBX1 gene, Tgfb2 gene, Heart development, Child

CHENG Fei，JIANG Hongkun. TBX1 gene may be involved in heart development and development of congenital heart disease through regulating Tgfb2 gene expression[J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2018, 10(1): 36-39.

[1]	. Expert consensus in integrated traditional Chinese and western medicine prevention and treatment for influenza in children [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(5): 369-.
[2]	NIU Zhenbing, LIU Jing, FENG Lili. Value of the combination detection of β2-microglobulin and neutrophil gelatinase-associated lipocalin in early renal injury in children with infectious mononucleosis [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(5): 399-.
[3]	LUO Lan, LIU Pingping, FANG Sisi. Study on resting energy expenditure of children with sepsis at acute stage [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(5): 405-.
[4]	TANG Xiaoli, DENG Hongbo. Retrospective analysis of the clinical characteristics of 23 children with pertussis [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(5): 416-.
[5]	XU Sha. Effect of pidotimod combined with vitamin AD on children with recurrent respiratory tract infection [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(5): 421-.
[6]	XIE Ying. Retrospective investigation of risk factors in hospitalized children with recurrent respiratory tract infection [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(5): 425-.
[7]	ZHANG Junqing, GUO Liang, SHI Cannan, WANG Jun. Changes and prediction analysis of the mortality of infants and children under 5 years old in Henan Province [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(5): 428-.
[8]	DU Linlin, WANG Yonghong, HE Jingwei. Study on the medication rules for cough in children [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(5): 431-.
[9]	DU Fuli, DAI Xiaoyu, ZHENG Yapei, ZHENG Hong. Discussion on the treatment of children's short stature based on time medicine of traditional Chinese medicine [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(5): 454-.
[10]	WANG Shengnan, WANG Guojie, WANG Hai. Observation on the effect of Weijing Ditan decoction in the treatment of children with protracted bacterial bronchitis [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(4): 281-.
[11]	HAN Jing, XU Yan, SUN Liping. Research progress in clinical application of Huaiqihuang granules in pediatrics [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(4): 292-.
[12]	WANG Shiyan, MA Bingxiang, LI Ruixing, XU Pingping. Research progress of tic disorder in children [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(4): 297-.
[13]	YUAN Jianzai, ZHANG Li, YUAN Yehong, MA Shuxia. Advances in research on the treatment for upper airway cough syndrome based on TCM syndrome differentiation [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(4): 301-.
[14]	LI Yi, SHEN Zhaobo, HUA Li. Observation on the efficacy of dust mite drops combined with terbutaline and montelukast in the treatment of allergic asthma in children [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(4): 325-.
[15]	LI Peng. Different doses of dexmedetomidineby nasal dripping in surgery for pediatric hernia [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(4): 328-.

TBX1 gene may be involved in heart development and development of congenital heart disease through regulating Tgfb2 gene expression

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments