Abstract:
objective:
To investigate the changes and clinical significance of the expression of P22phox mRNA of peripheral blood mononuclear cells(PBMCs) in children with Kawasaki disease(KD) before and after treatment.
Methods:
Totally 40 Kawasakai disease patients admitted to Pediatric Department of Northern Sichuan Medical College Affiliated Hospital from October 2015 to June 2016（15 patients with coronary artery lesions,25 patients without coronary artery lesions） who received IVIG treatment were selected in the study, and 20 age-matched healthy children undergoing elective surgery in the Pediatric Surgery of this hospital were selected as the control group. The expression of P22phox mRNA of the peripheral blood mononuclear cells(PBMCs) were measured using real-time polymerase chain reaction(RT-PCT) in the two groups before and after treatment.
Results:
In the treatment group, there was statistical difference in P22phox mRNA expression before and after treatment(P<0.05); the expression of P22phox mRNA had statistical difference between treatment group before treatment and the control group(P<0.01);there was no statistical difference in P22phox mRNA expression between treatment group after treatment and the control group(P>0.05). The expression of P22phox mRNA was not statistically different in the following cases: with or without coronary lesion before treatment with or without coronary lesion after treatment, with coronary lesion before or after treatment, and without coronary lesion before or after treatment(P>0.05).
Conclusion:P22phox may be involved in the pathogenesis of Kawasaki disease at acute stage.

Key words: Kawasaki disease, P22phox, Coronary artery lesions, No coronary artery lesion, Child