Abstract:
Objective
To detect whether the serum of the rats irrigated with rhubarb and leech contain the main components of these two drugs, and to provide theoretical basis for clinical use of drugs.
Methods
The effective components, rhein, emodin and hypoxanthine, in serum of rhubarb group and leech group were determined by HPLC.
Results
The rat serum of rhubarb group contained rhein and emodin;although the detection of rhein had certain interference, emodin could be detected. Hypoxanthine could be detected in the rat serum of leech group.
ConclusionIt is confirmed that the main chemical constituents of rhubarb and leech are found in rat serum.

Key words: High performance liquid chromatography, Rhein, Emodin, Hypoxanthine