Abstract:
Objective：To explore mechanism of prevention and treatment of  Double Relieving Superficies Syndrome Methods by investigating the expression of TLR4-MyD88-NF-κB P65 protein on influenza viral  pneumonia in mice.Methods：The mice were infected with influenza virus. The traditional Chinese medicine groups were provided for Yinqiao power，Yupingfeng power and Yinqiao power+Yupingfeng power. The control group was provided for ribavirin.Then expression of TLR4-MyD88-NF-κB P65 protein was measured by SABC immunohistochemical assay after five days.Results：The protein expression of TLR4-MyD88-NF-κB P65 in Double Relieving Superficies Syndrome Methods group was lower than that of other groups(P＜0.05).Conclusion：Influenza virus can trigger the TLR4-MyD88-NF-κB P65 message channel. Double Relieving Superficies Syndrome Methods can suppress the expression of TLR4-MyD88-NF-κB P65 protein and is better than others.

Key words: Double Relieving Superficies Syndrome Methods；TLR4-MyD88-NF-κB P65;mice;animal, experiment