中药二步序贯治疗对哮喘小鼠肺组织GATA-3表达及气道炎症的影响

doi:10.3969/j.issn.1674-3865.2009.04.002

Abstract

Abstract: Objective：To study the effect of Xiaoqinglong decoction and Ginseng-Schisandrae decoction on the expression of GATA-3 and airway inflammation in lungs of mice with asthma.Methods：Thirty mice were randomly divided into three groups(n=10,in each group):normal control group,model group,Xiaoqinglong decoction and Ginseng-Schisandrae decoction group.The mouse group of asthma were made by the egg albumen sensitization method and treated early with Xiaoqinglong decoction and Ginseng-Schisandrae decoction.OVA-IgE in the serum of mice were detected with enzyme-linked immunosorbent assay (ELISA).The total number of cells in the BALF and the classification of cells were tested.The pathology of lung tissue was examined by HE.GATA-3 in the lung tissue was examined with immunohistochemical method.Results：The OVA-IgE and GATA-3 were decreased in the Xiaoqinglong decoction and Ginseng-Schisandrae decoction group compared with the model group and was increased compared with the normal control group.The total cells and the percentage of eosinophils and lymphocytes in BALF in Xiaoqinglong decoction and Ginseng-Schisandrae decoction group were decreased compared with those in model group and increased compared with those in normal control group(all P＜0.01).The percentage of phagocyte in BALF in Xiaoqinglong decoction and Ginseng-Schisandrae decoction group was increased compared with those in model group and decreased compared with those in normal control group(P＜0.01).Conclusion：Xiaoqinglong decoction and Ginseng-Schisandrae decoction may regulate immune system by influencing the expression of GATA-3,which plays an important role in treating asthmatic mice.

Key words: Asthma, Mice, GATA-Ginseng-Schisandrae decoction, Xiaoqinglong decoction, Asthma/treatment, Animal,experiment

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