ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2014, Vol. 6 ›› Issue (3): 203-205.doi: 10.3969/j.issn.1674-3865.2014.03.005

• 儿童多动症专栏 • 上一篇    下一篇

龙牡桂枝汤改善注意缺陷多动障碍模型大鼠行为的实验研究

何平，唐彦，杨若俊   

  1. 650021 昆明，云南中医学院第一附属医院儿科（何平）；云南中医学院儿科教研室（唐彦，杨若俊）
  • 出版日期:2014-06-25 发布日期:2018-11-19
  • 通讯作者: 唐彦，650021 昆明，云南中医学院儿科教研室。
  • 作者简介:何平（1964-），女，副主任医师。研究方向：小儿心肝疾病的中医研究。
  • 基金资助:
    云南省科技厅自然科学基金面上项目（2010CD074）

Behavioral study of Longmu Guizhi decoction on ADHD model rats  

HE Ping，TANG Yan,YANG Ruo jun.     

  1. Yunnan University of Traditional Chinese Medicine,Kunming 650021，China.
  • Online:2014-06-25 Published:2018-11-19

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摘要：
目的：观察龙牡桂枝汤对注意缺陷多动障碍(ADHD)模型动物——幼年自发性高血压大鼠(SHR)的自发活动和非选择性注意力的影响。
方法：采用幼年雄性SHR大鼠48只，随机分为龙牡桂枝汤高、中剂量组、盐酸哌甲酯组和生理盐水组，连续灌胃给药14 d。通过开场实验和Làt迷宫实验分别检测给药前后大鼠自发活动和非选择性注意力的变化。
结果：在开场实验中，各药物组穿越格子数和直立次数明显少于生理盐水组，差异有统计学意义(P<0.01)；盐酸哌甲酯组大鼠穿越格子数和直立次数少于中药中剂量组，差异有统计学意义(P<0.05)，而中药高剂量组与盐酸哌甲酯组比较差异无统计学意义（P>0.05)。Làt迷宫实验中，各药物组斜搭次数多于生理盐水组，差异有统计学意义(P<0.01)，中药高剂量组的斜搭次数多于中剂量组及盐酸哌甲酯组，差异有统计学意义(P<0.05)。
结论：龙牡桂枝汤可改善ADHD模型大鼠的自发活动和非选择性注意力，并呈现出一定的量效关系。

关键词: 轻微脑损伤综合征, 龙牡桂枝汤, 动物, 实验, 大鼠

Abstract:
Objective:To investigate the effect of Longmu Guizhi decoction(LD) on locomotor activity and nonselective attention of juvenile SHR(spontaneously hypertensive rat)——a rat model of attention deficit hyperactivity disorder.
Methods:Forty-eight SHR male  were randomly divided into high-dose LD group, middle-dose LD group, methylphenidate control group and saline control group. All the SHRs were lavaged for 14 days. Locomotor activity and nonselective attention were examined by openfield test and Làt maze test respectively before and after intervention.
Results :In openfield test ,the number of square crossing and rearing after intervention of methylphenidate and decoctions in SHRs was significantly fewer than the saline group (P<0.01). The number of the above items in methylphenidate group was significantly fewer than middle-dose LD group (P<0.05). There was no difference between methylphenidate group and high-dose LD group（P>0.05).In Làt maze test, frequency of leaning against the wall after intervention of methylphenidate and decoctions in SHRs was significantly increased compared with the saline group (P<0.01), and that of high-dose LD group was significantly higher than the methylphenidate group and middledose LD group(P<0.05).
Conclusion :Longmu Guizhi decoction can reduce locomotor activity and improve non-selective attention of ADHD model rats,which presents some kind of dose-effect relationship.

Key words: mild brain injury syndrome, Longmu Guizhi decoction, animal, experiment, rat