Chinese Pediatrics of Integrated Traditional and Western Medicine

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25 June 2015, Volume 7 Issue 3 Previous Issue Next Issue

Study on the effect of early hyperbaric oxygen in protecting brain nerves of neonatal rats with hypoxic ischemic brain damage

JIANG Tao,BAO Jing，ZHANG Lihua，BAO Xiuqin

2015, 7 (3): 215-218. doi: 10.3969/j.issn.1674-3865.2015.03.007

Abstract ( 427 )

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Objective：To investigate the effect of early hyperbaric oxygen(HBO) in protecting brain nerves of neonatal rats with hypoxic ischemic brain damage(HIBD).
Methods：A total of 120 7-day old neonatal Wistar rats were randomly divided into sham operation group(control group), hypoxic ischemic brain injury group(group HIBD) and hyperbaric oxygen group(HBO group), with 40 rats in each group. The HBO group was randomly divided into early hyperbaric oxygen subgroups(EHBO subgroup) and late hyperbaric oxygen subgroups(LHBO subgroup),with 20 rats in each group. After establishing the HIBD model for all rats(control group with sham operation),hyperbaric oxygen treatment was performed in EHBO subgroup after 1 h, and in LHBO subgroup after 48 h(2 ATA, every 90 minutes, 24 hours once, continuous treatment of 7 day).After treatment, the rats in subgroups were killed, and the same number of rats in the control group and HIBD group were also killed. Hippocampus Nestin in the nerve of rat was determined using immunohistochemical method, and learning and memory function in rats were tested using the Morris water maze test.
Results：In the control group Nestin positive cell detection rate was (10.26±1.37)/HP, in EHBO subgroup it was (15.82±2.19)/HP, LHBO subgroup (32.64±4.83)/HP, HIBD group (36.18±4.02)/HP. The detection rate of Nestin positive cells in the HBO group, and HIBD group was significantly higher than that of the control group(P<0.05), and in the LHBO subgroup it was significantly higher compared with that of the EHBO subgroups(P<0.05), while the LHBO subgroup and HIBD group had no significant difference(P>0.05); the four groups had significant differences in average EL (F=24.18, P<0.05);the rats in control group，EHBO and LHBO group had significant difference in the search time and search distance(F=21.05, P<0.05), but there was no significant difference between LHBO and HIBD groups and among the groups in search time(P>0.05).
Conclusion：Early BHO has protective effect on brain injury;Nestin protein may be involved in the repair of brain nerves;early HBO can improve the future learning and memory function which is impaired by HIBD.

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The expression and significance of β-Catenin，transforming growth factor beta 1(TGF-beta 1), Clara cell secretory protein 16(cc16) and surface antigen 6 of alveolar epithelial cell Ⅱ(KL-6) in bronchialveolar lavage fluid(BALF) of children with bronchial pulmonary dysplasia(BPD)

YANG Min，LI Linrui，HUANG Jianbao，MENG Yanni，CHEN Yanping

2015, 7 (3): 219-223. doi: 10.3969/j.issn.1674-3865.2015.03.008

Abstract ( 353 )

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Objective： To investigate the expression of β-Catenin，transforming growth factor beta 1(TGF-beta 1), Clara cell secretory protein 16 (cc16), and surface antigen 6 of alveolar epithelial cell Ⅱ(KL-6) in bronchoalveolar lavage fluid(BALF) of children with bronchial pulmonary dysplasia(BPD) and its significance.
Method : Of 70 cases of premature infants with mechanical ventilation, 40 cases diagnosed with BPD were included in observation group, 30 cases of non-BPD patients were as control group. Detect the levels of β-Catenin, TGF-beta 1, cc16 and KL-6 in BALF of two groups of children respectively after 1, 24, 48 and 72 h of mechanical ventilation using enzyme-linked immunosorbent assay method.
Result : Average gestational age and birth body mass of observation group were significantly lower than those of the control group, the difference being statistically significant (P＜0.05).The time of mechanical ventilation and oxygen therapy were significantly longer than the control group, the difference being statistically significant(P＜0.01). With longer duration of mechanical ventilation, the levels of cc16 of observation group showed a downward trend, and β-Catenin, TGF-beta 1 and KL-6 levels showed an upward trend, and the level difference of each index in time point of 48 and 72 h was statistically significant compared with that of 1 h(P＜0.01). The difference of the level of TGF-beta 1 and cc16 of control group at time point 1 h and 72 h of mechanical ventilation was statistically significant (P＜0.01), but the differences of level of beta protein and KL-6 at various time points had no statistical significance (P>0.05). Index levels of β-Catenin and TGF-beta 1 of observation group with mechanical ventilation at each time point were significantly higher than control group in the corresponding time point, but cc16 was significantly lower than the control group, the difference being statistically significant (P＜0.05), while the KL-6 of observation group was higher than the control group in the corresponding time point of 48 and 72 h, the difference being statistically significant(P＜0.05).There was no statistically significant difference at other time points(P>0.05). Comparing each time point,β-catenin, TGF-β1 and KL-6 levels in the moderate and severe BPD group were significantly higher than mild group (P＜0.05), but cc16 level was significantly lower than mild group (P＜0.05). Multiple stepwise regression analysis results showed that the longer duration of mechanical ventilation time and oxygen and the low cc16 levels are a risk factor for the occurrence of BPD(P＜0.01).
Conclusion： The occurrence of premature BPD is closely related to longer duration of mechanical ventilation and oxygen and low cc16 levels. Monitoring the level of β-Catenin, TGF-beta 1 and cc16 of BALF of premature infants in initial mechanical ventilation can provide the basis for predicting the occurrence of premature BPD and its classification.