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25 April 2013, Volume 5 Issue 2 Previous Issue    Next Issue

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The effect of intravenous immune globulin on Tolllike receptor 4 expression of brain tissues in fetal rats with intrauterine infection. DAIMengying,LI Xiaojie,SUN Qifeng. 2013, 5 (2):  118-121.  doi: 10.3969/j.issn.1674-3865.2013.02.011 Abstract ( 262 )   PDF (486KB) ( 88 )   Save Objective:To observe the effect of IVIG on Tolllike receptor 4 expression of brain tissues exposed on perinatal inflammation.MethodsForty pregnant SD rats were randomly divided into three groups：intervention group (LPS + IVIG group)(16 rats)，in the 17thday of the conception,give them LPS intraperitoneal injection，with the dose of 350 μg/kg. Give 2 g/kgimmune globulin injection from the female rats' tail vein three hours after the models were made;experimental group (LPS group)(16 rats)，use the same way to make intrauterine infection model of pregnant rats;Control group (NS group)(8 rats)，give intraperitoneal injection of normal saline at the 17thday of gestation. Take intraperitoneal injection of 24 hours (pregnant day 18)，pregnant day 19，pregnant day 21，and the day after birth. Observe the placenta and the point of time of the pathological changes of the brain tissue with hematoxylineosin staining;compare the weight of the fetal rat; PCR was used to measure the TLR4mRNA content; immune staining was used to observe each group's TLR4 expression at each time point. ResultsIn the experimental group, pregnant rats placenta and uterine wall had congestion and edema，and there was leukocyte infiltration，white matter edema and morphology of naive cells，or even visible focal hemorrhage.In experimental group and intervention group offspring weight had varying degrees of loss，there being significant differences compared to control group(P＜0.01).In experimental group and the intervention group's offspring brain，the TLR4mRNA and TLR4 expression volume are both increased at different time point.ConclusionIntrauterine LPS infection can lead to fetal brain tissue inflammatory response and giving IVIG timely can reduce the sustained activation of microglia,which inhibits fetal brain tissue inflammation，thereby reducing the extent of brain damage，and achieve the effect of brain protection. Related Articles | Metrics

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Replication of sodium valproateinduced animal model of autism. CUI Lijun，GUO Lanmin，GUO Jin，et al. 2013, 5 (2):  122-125.  doi: 10.3969/j.issn.1674-3865.2013.02.012 Abstract ( 277 )   PDF (497KB) ( 147 )   Save Objective:To investigate the replication method of sodium valproateinduced animal model of autism.Methods:Femalerats received a single intraperitoneal injection of sodium valproate(600 mg/kg) on the 12.5 day after conception in the experiment，the pups as model group;and control females were injected with physiological saline at the same time，the pups as control group. Sodium valproate was dissolved in saline at a concentration of 250 g/L. Observe and test the physical development，social interaction，communication and stereotyped，repetitive patterns of behaviors of the pups. Test physical development and social interaction and communication and physical contact with companion.Results:Compared with control group，model group rats showed lower body weight，delayed open eyes，attenuated coordination of direction and swimming，lower levels of following and pushcrawl and higher levels of selfgrooming，and lower levels of sniffs of the cotton swab of rat urine.Conclusion:Theoperation of replicate sodium valproateinduced animal model of autism is simple. The success rate of rat model is high. Related Articles | Metrics